Proteintech

CD200 Monoclonal Antibody (5F3D6)

CD200 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD200 Antibody (66282-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 66282-1-IG (CD200 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

66282-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5F3D6

Immunogen

CD200 Fusion Protein Ag5045 (1-269 aa encoded by BC022522)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.63 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MERLVIRMP FCHLSTYSLV WVMAAVVLCT AQVQVVTQDE REQLYTPASL KCSLQNAQEA LIVTWQKKKA VSPENMVTFS ENHGVVIQPA YKDKINITQL GLQNSTITFW NITLEDEGCY MCLFNTFGFG KISGTACLTV YVQPIVSLHY KFSEDHLNIT CSATARPAPM VFWKVPRSGI ENSTVTLSHP NGTTSVTSIL HIKDPKNQVG KEVICQVLHL GTVTDFKQTV NKGYWFSVPL LLSIVSLVIL LVLISILLYW KRHRNQDREP (1-269 aa encoded by BC022522)

Target Information

CD200 is a type-1 membrane glycoprotein in the immunoglobulin superfamily, featuring two immunoglobulin domains. It is widely expressed on various cells, including B and T cells, keratinocytes, nerve cells, follicular dendritic cells, ovarian cells, and trophoblasts. CD200 interacts with CD200R on myeloid cells, inhibiting macrophage activation, reducing IL-6 production, and attenuating lymphocyte activation. It also prevents mast cell degranulation and decreases TNF-alpha and IL-13 secretion, playing a role in immune regulation and maternal tolerance. Homologues of CD200 are found in herpesviruses and poxviruses, indicating a conserved immune modulation function. CD200 has multiple alternatively spliced transcript variants, contributing to its functional diversity. Diseases linked to CD200 dysfunction include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy, highlighting its importance in health and disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Multiplex Quantitative Analysis of Tumor-Infiltrating Lymphocytes, Cancer-Associated Fibroblasts, and CD200 in Pancreatic Cancer.

Cancers

MacNeil T,Vathiotis IA,Shafi S,Aung TN,Zugazagoitia J,Gruver AM,Driscoll K,Rimm DL

Tue Nov 02 00:00:00 UTC 2021

CD200 and CD200R1 are differentially expressed and have differential prognostic roles in non-small cell lung cancer.

Oncoimmunology

Yoshimura K,Suzuki Y,Inoue Y,Tsuchiya K,Karayama M,Iwashita Y,Kahyo T,Kawase A,Tanahashi M,Ogawa H,Inui N,Funai K,Shinmura K,Niwa H,Sugimura H,Suda T

Wed Jul 28 00:00:00 UTC 2021

CD200 and CD200R1 are differentially expressed and have differential prognostic roles in non-small cell lung cancer.

Oncoimmunology

Yoshimura K,Suzuki Y,Inoue Y,Tsuchiya K,Karayama M,Iwashita Y,Kahyo T,Kawase A,Tanahashi M,Ogawa H,Inui N,Funai K,Shinmura K,Niwa H,Sugimura H,Suda T

Wed Jul 28 00:00:00 UTC 2021

CD200 and CD200R1 are differentially expressed and have differential prognostic roles in non-small cell lung cancer.

Oncoimmunology

Yoshimura K,Suzuki Y,Inoue Y,Tsuchiya K,Karayama M,Iwashita Y,Kahyo T,Kawase A,Tanahashi M,Ogawa H,Inui N,Funai K,Shinmura K,Niwa H,Sugimura H,Suda T

Wed Jul 28 00:00:00 UTC 2021

CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors.

Scientific reports

Ishibashi M,Neri S,Hashimoto H,Miyashita T,Yoshida T,Nakamura Y,Udagawa H,Kirita K,Matsumoto S,Umemura S,Yoh K,Niho S,Tsuboi M,Masutomi K,Goto K,Ochiai A,Ishii G

Fri Apr 21 00:00:00 UTC 2017

CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors.

Scientific reports

Ishibashi M,Neri S,Hashimoto H,Miyashita T,Yoshida T,Nakamura Y,Udagawa H,Kirita K,Matsumoto S,Umemura S,Yoh K,Niho S,Tsuboi M,Masutomi K,Goto K,Ochiai A,Ishii G

Fri Apr 21 00:00:00 UTC 2017

CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors.

Scientific reports

Ishibashi M,Neri S,Hashimoto H,Miyashita T,Yoshida T,Nakamura Y,Udagawa H,Kirita K,Matsumoto S,Umemura S,Yoh K,Niho S,Tsuboi M,Masutomi K,Goto K,Ochiai A,Ishii G

Fri Apr 21 00:00:00 UTC 2017

CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors.

Scientific reports

Ishibashi M,Neri S,Hashimoto H,Miyashita T,Yoshida T,Nakamura Y,Udagawa H,Kirita K,Matsumoto S,Umemura S,Yoh K,Niho S,Tsuboi M,Masutomi K,Goto K,Ochiai A,Ishii G

Fri Apr 21 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody MRC OX-2; CD200; CD200 antigen; OX-2 membrane glycoprotein

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Gene Aliases: CD200; MOX1; MOX2; MRC; My033; OX-2

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UniProt ID: (Human) P41217

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Entrez Gene ID: (Human) 4345

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Function(s)

receptor activity receptor binding protein binding protein homodimerization activity cell adhesion molecule binding

Process(es)

homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules cell recognition negative regulation of macrophage activation regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD200 Monoclonal Antibody (5F3D6)

CD200 Antibody (66282-1-IG) in IHC (P)

Product Details

66282-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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