Invitrogen

CD3 Monoclonal Antibody (SK7), PerCP-eFluor™ 710, eBioscience™

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CD3 Antibody (46-0036-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD3 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone SK7 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes (middle plot). Additional analysis of lymphocytes shows that CD3 clone SK7 does not stain any CD19+ B cells (right plot). Details: Normal human whole blood was surface stained with CD3 (clone SK7) and CD19 (clone HIB19). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used to compare CD3 staining (middle plot). Cells in the lymphocyte gate were used to compare CD3 and CD19 staining (right plot). {RE}

Product Details

46-0036-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Chimpanzee, Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SK7

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2043833

Product Specific Information

Description: The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK7 and UCHT1 monoclonal antibodies cross-block binding, suggesting recognition of overlapping epitope. In contrast clones OKT3 and SK7 see different epitopes.

The antibody SK7 recognizes chimpanzee CD3.

Applications Reported: This SK7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SK7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer.

The Journal of biological chemistry

Cha JH,Chan LC,Wang YN,Chu YY,Wang CH,Lee HH,Xia W,Shyu WC,Liu SP,Yao J,Chang CW,Cheng FR,Liu J,Lim SO,Hsu JL,Yang WH,Hortobagyi GN,Lin C,Yang L,Yu D,Jeng LB,Hung MC

Published figure using CD3 monoclonal antibody (Product # 46-0036-42) in Flow Cytometry

Fri Apr 01 00:00:00 UTC 2022

Direct and indirect immune effects of CMP-001, a virus-like particle containing a TLR9 agonist.

Journal for immunotherapy of cancer

Sabree SA,Voigt AP,Blackwell SE,Vishwakarma A,Chimenti MS,Salem AK,Weiner GJ

Published figure using CD3 monoclonal antibody (Product # 46-0036-42) in Flow Cytometry

Tue Jun 01 00:00:00 UTC 2021

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using CD3 monoclonal antibody (Product # 46-0036-42) in Flow Cytometry

Mon Sep 28 00:00:00 UTC 2020

Synthetic Abortive HIV-1 RNAs Induce Potent Antiviral Immunity.

Frontiers in immunology

Stunnenberg M,Sprokholt JK,van Hamme JL,Kaptein TM,Zijlstra-Willems EM,Gringhuis SI,Geijtenbeek TBH

Published figure using CD3 monoclonal antibody (Product # 46-0036-42) in Flow Cytometry

Fri Feb 19 00:00:00 UTC 2021

Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer.

BMC cancer

Choi JW,Lee ES,Kim SY,Park SI,Oh S,Kang JH,Ryu HA,Lee S

Published figure using CD3 monoclonal antibody (Product # 46-0036-42) in Flow Cytometry

Mon Aug 19 00:00:00 UTC 2019

Identification and characterization of an alternative cancer-derived PD-L1 splice variant.

Cancer immunology, immunotherapy : CII

Hassounah NB,Malladi VS,Huang Y,Freeman SS,Beauchamp EM,Koyama S,Souders N,Martin S,Dranoff G,Wong KK,Pedamallu CS,Hammerman PS,Akbay EA

46-0036-42 was used in Flow Cytometry to demonstrate that truncated forms of PD-L1 exist in numerous cancer types, and have validated that truncated PD-L1 can be secreted and negatively regulate T cell function.

Fri Mar 01 00:00:00 UTC 2019

Psoriasis patients demonstrate HLA-Cw*06:02 allele dosage-dependent T cell proliferation when treated with hair follicle-derived keratin 17 protein.

Scientific reports

Yunusbaeva M,Valiev R,Bilalov F,Sultanova Z,Sharipova L,Yunusbayev B

Published figure using CD3 monoclonal antibody (Product # 46-0036-42) in Flow Cytometry

Tue Apr 17 00:00:00 UTC 2018

Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.

Frontiers in pharmacology

Guo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z

Published figure using CD3 monoclonal antibody (Product # 46-0036-42) in Flow Cytometry

Thu Sep 28 00:00:00 UTC 2023

IFN-λ3 polymorphism indirectly influences NK cell phenotype and function during acute HCV infection.

Immunity, inflammation and disease

Depla M,Pelletier S,Bédard N,Brunaud C,Bruneau J,Shoukry NH

46-0036 was used in Flow cytometry/Cell sorting to study how polymorphisms in the type III interferon, IFN-λ3, can determine the outcome of HCV infection.

Thu Sep 01 00:00:00 UTC 2016

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD3 monoclonal antibody (Product # 46-0036-42) in Flow Cytometry

Fri Oct 13 00:00:00 UTC 2017

A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Kap YS,van Meurs M,van Driel N,Koopman G,Melief MJ,Brok HP,Laman JD,'t Hart BA

Tue Dec 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD3 Monoclonal Antibody (SK7), PerCP-eFluor™ 710, eBioscience™

CD3 Antibody (46-0036-42) in Flow

Product Details

46-0036-42

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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