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Invitrogen
CD300e (IREM-2) Monoclonal Antibody (UP-H2), APC, eBioscience™
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FIGURE: 1 / 1
CD300e (IREM-2) Antibody (17-3007-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD85d (ILT4) FITC (Product # 11-5148-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD300e (IREM-2) APC (right). Cells in the monocyte gate were used for analysis.
Product Details
17-3007-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
UP-H2
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11219878
Product Specific Information
Description: The UP-H2 monoclonal antibody recognizes human IREM-2 (immune receptor expressed in monocytic derived cells) also known as LMIR6, and unofficially CD300E and CD300LE. It is a member of the CMRF-35 family. This 34 kDa Ig-like containing receptor is an activating receptor and through it's charged residues in the cytokplasmic domain interacts with ITAM containing adaptor proteins like DAP12. Expression is found exclusively on monocytes and myeloid DC's. Expression may be transient as differentiation of monocytes leads to down regulation of IREM-2 yet expression is found on mature DCs. It has been also noted that myeloid cell lines such as THP-1 and U937 do not express IREM-2.
In vitro addition of UP-H2 antibody either soluble or crosslinked stimulates monocytes.
Applications Reported: This UP-H2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This UP-H2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD300e is a member of the glycoprotein family of cell surface proteins expressed on myeloid cells. CD300e interacts with the TYRO protein tyrosine kinase-binding protein and is thought to be involved as an activating receptor. CD300e is expressed on mature myeloid dendritic cells and monocytes, and is down regulated on immature dendritic cells. Further, CD300e interacts with DAP-12, which mediates activating signals. The alloreactive response of naïve T cells has been reported to become enhanced by the activation of CD300e. Diseases associated with CD300e dysfunction include tungiasis and lice infestation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD300 antigen like family member E; CD300 antigen-like family member E; CD300e; CD300e antigen; CLM-2; CMRF35-A5; CMRF35-like molecule 2; Immune receptor expressed on myeloid cells 2; IREM-2; PIgR-2; poly-Ig receptor 2; Polymeric immunoglobulin receptor 2
Gene Aliases: CD300E; CD300LE; CLM-2; CLM2; CMRF35-A5; CMRF35A5; IREM-2; IREM2; PIgR-2; PIgR2
UniProt ID: (Human) Q496F6
Entrez Gene ID: (Human) 342510
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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