Invitrogen

CD31 Monoclonal Antibody (ER-MP12)

CD31 Antibody in Immunocytochemistry (ICC/IF)

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CD31 Antibody (MA1-40074) in ICC/IF

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2021 - 3 Figure TSP2 is expressed in perivascular, but not endothelial cells. Transverse femoral fractures were induced in TSP2-GFP reporter mice. Femurs were harvested prior to fracture and on day 1, 3, 5, 7, and 14 post-fracture. A representative day 5 fracture is shown in the figure. The selected region is from the midline of the callus along the midline column of high T2GFP expression (see Figure 2B). A, Repre... View More

CD31 Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD31 Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-40074

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Immunohistochemistry (Frozen) (IHC (F))

1:50

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50

ELISA (ELISA)

1:50

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

ER-MP12

Immunogen

Murine macrophage precursor hybrid W1C3cell hybrid

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1072120

Product Specific Information

MA1-40074 detects CD31 from mouse samples.
MA1-40074 reacts with an antigen present on cell subpopulations of adult mouse bone marrow, including pluripotent hematopoietic stem cells. MA1-40074 reacts with the major population of colony forming unit macrophage (CFU-M) precursor cells, with subpopulation of pre CFU-M and monoblasts, and with precursor cells of granulocytes.

MA1-40074 has been successfully used in ELISA, immunohistochemistry (paraffin and frozen), immunoprecipitation, and flow cytometry applications. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting, working dilution is 1:50.

The MA1-40074 immunogen is mouse PECAM-1/CD31.

Suggested positive control: Macrophage cell line RMB-1.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

High affinity of β-amyloid proteins to cerebral capillaries: implications in chronic lead exposure-induced neurotoxicity in rats.

Fluids and barriers of the CNS

Liu LL,Shen X,Gu H,Zhao G,Du Y,Zheng W

MA1-40074 was used in Immunohistochemistry to demonstrate that the cerebral vasculature naturally possessed a high affinity to Aβ present in circulating blood.

Mon May 01 00:00:00 UTC 2023

High affinity of β-amyloid proteins to cerebral capillaries: implications in chronic lead exposure-induced neurotoxicity in rats.

Fluids and barriers of the CNS

Liu LL,Shen X,Gu H,Zhao G,Du Y,Zheng W

MA1-40074 was used in Immunohistochemistry to demonstrate that the cerebral vasculature naturally possessed a high affinity to Aβ present in circulating blood.

Mon May 01 00:00:00 UTC 2023

Old age promotes retinal fibrosis in choroidal neovascularization through circulating fibrocytes and profibrotic macrophages.

Journal of neuroinflammation

Yi C,Liu J,Deng W,Luo C,Qi J,Chen M,Xu H

MA1-40074 was used in ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation to investigate the effect of ageing on subretinal fibrosis secondary to choroidal neovascularization and the mechanism of action.

Thu Feb 23 00:00:00 UTC 2023

Thrombospondin-2 spatiotemporal expression in skeletal fractures.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Zondervan RL,Jenkins DC,Reicha JD,Hankenson KD

MA1-40074 was used in Immunohistochemistry (Frozen) to suggest that TSP2 is expressed by undifferentiated MSCs, but downregulated in chondrocytes.

Fri Jan 01 00:00:00 UTC 2021

Thrombospondin-2 spatiotemporal expression in skeletal fractures.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Zondervan RL,Jenkins DC,Reicha JD,Hankenson KD

MA1-40074 was used in Immunohistochemistry (Frozen) to suggest that TSP2 is expressed by undifferentiated MSCs, but downregulated in chondrocytes.

Fri Jan 01 00:00:00 UTC 2021

Thrombospondin-2 spatiotemporal expression in skeletal fractures.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Zondervan RL,Jenkins DC,Reicha JD,Hankenson KD

MA1-40074 was used in Immunohistochemistry (Frozen) to suggest that TSP2 is expressed by undifferentiated MSCs, but downregulated in chondrocytes.

Fri Jan 01 00:00:00 UTC 2021

Increased vascular permeability and severe renal tubular damage after ischemia-reperfusion injury in mice lacking adiponectin or T-cadherin.

American journal of physiology. Endocrinology and metabolism

Tsugawa-Shimizu Y,Fujishima Y,Kita S,Minami S,Sakaue TA,Nakamura Y,Okita T,Kawachi Y,Fukada S,Namba-Hamano T,Takabatake Y,Isaka Y,Nishizawa H,Ranscht B,Maeda N,Shimomura I

MA1-40074 was used in Immunohistochemistry-immunofluorescence to suggest that APN protected the kidney in an acute renal injury model by binding to T-cadherin.

Mon Feb 01 00:00:00 UTC 2021

Reduction of mNAT1/hNAT2 Contributes to Cerebral Endothelial Necroptosis and Aβ Accumulation in Alzheimer's Disease.

Cell reports

Zou C,Mifflin L,Hu Z,Zhang T,Shan B,Wang H,Xing X,Zhu H,Adiconis X,Levin JZ,Li F,Liu CF,Liu JS,Yuan J

MA1-40074 was used in Immunohistochemistry to show that venular and capillary cerebral endothelial cells (ECs) are selectively vulnerable to necroptosis in AD.

Tue Dec 08 00:00:00 UTC 2020

Expansion of BCR/ABL1⁺ cells requires PAK2 but not PAK1.

British journal of haematology

Edlinger L,Berger-Becvar A,Menzl I,Hoermann G,Greiner G,Grundschober E,Bago-Horvath Z,Al-Zoughbi W,Hoefler G,Brostjan C,Gille L,Moriggl R,Spittler A,Sexl V,Hoelbl-Kovacic A

Published figure using CD31 monoclonal antibody (Product # MA1-40074) in Immunohistochemistry

Sun Oct 01 00:00:00 UTC 2017

Endothelin A receptor activation on mesangial cells initiates Alport glomerular disease.

Kidney international

Dufek B,Meehan DT,Delimont D,Cheung L,Gratton MA,Phillips G,Song W,Liu S,Cosgrove D

Published figure using CD31 monoclonal antibody (Product # MA1-40074) in Immunofluorescence

Mon Aug 01 00:00:00 UTC 2016

Lymph node stromal cells constrain immunity via MHC class II self-antigen presentation.

eLife

Baptista AP,Roozendaal R,Reijmers RM,Koning JJ,Unger WW,Greuter M,Keuning ED,Molenaar R,Goverse G,Sneeboer MM,den Haan JM,Boes M,Mebius RE

MA1-40074 was used in immunohistochemistry to examine the role of lymph node stromal cells in immune responses

Wed Nov 19 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: CD31; PECAM-1; Platelet endothelial cell adhesion molecule

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Gene Aliases: C85791; Cd31; Pecam; PECAM-1; Pecam1

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UniProt ID: (Mouse) Q08481

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Entrez Gene ID: (Mouse) 18613

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD31 Monoclonal Antibody (ER-MP12)

CD31 Antibody (MA1-40074) in ICC/IF

Product Details

MA1-40074

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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