Invitrogen

CD43 Monoclonal Antibody (eBioR2/60), Biotin, eBioscience™

8 Published Figures

19 References

View all (111) CD43 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

FIGURE: 1 / 9

CD43 Antibody (13-0431-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgM kappa Isotype Control Biotin (Product # 13-4341-81) (left) or 0.25 µg of Anti-Mouse CD43 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

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Product Details

13-0431-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 17 publications 17 publications

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgM

Recommended Isotype Control

Rat IgM Isotype Control (eBRM), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioR2/60

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_466439

Product Specific Information

Description: The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival.

While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.

Applications Reported: This eBioR2/60 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioR2/60 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD43, also known as leukosialin or sialophorin, is a transmembrane mucin-like protein characterized by a high negative charge. It is expressed on the surface of most hematopoietic cells, excluding resting B lymphocytes. CD43 serves both adhesive and anti-adhesive functions, contributing to a repulsive barrier that can interfere with cellular adhesion while also promoting leukocyte aggregation in certain contexts. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. Its expression decreases upon activation due to proteolytic processing. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages. CD43 interacts with actin-binding proteins ezrin and moesin, playing a regulatory role in remodeling T-cell morphology and regulating cell-cell interactions during lymphocyte traffic. It enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors. CD43 signaling induces the functionally active tumor suppressor p53 protein, but in cases of p53 and ARF deficiency, it can promote tumor proliferation and viability. CD43 is an important modulator of leukocyte functions, with dysfunctions associated with diseases such as Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Progressive differentiation toward the long-lived plasma cell compartment in the bone marrow.

The Journal of experimental medicine

Koike T,Fujii K,Kometani K,Butler NS,Funakoshi K,Yari S,Kikuta J,Ishii M,Kurosaki T,Ise W

13-0431-82 was used in Magnetic cell separation to suggest that the adhesion status of bone marrow plasma cells is dynamically altered during their differentiation and is associated with provision of survival signals.

Mon Feb 06 00:00:00 UTC 2023

WASp Is Crucial for the Unique Architecture of the Immunological Synapse in Germinal Center B-Cells.

Frontiers in cell and developmental biology

Li Y,Bhanja A,Upadhyaya A,Zhao X,Song W

13-0431-82 was used in Flow Cytometry to reveal essential roles for WASp in the generation and maintenance of unique structures for GCB immunological synapses.

Mon Feb 28 00:00:00 UTC 2022

The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition.

Cell reports

Azzoni E,Frontera V,Anselmi G,Rode C,James C,Deltcheva EM,Demian AS,Brown J,Barone C,Patelli A,Harman JR,Nicholls M,Conway SJ,Morrissey E,Jacobsen SEW,Sparrow DB,Harris AL,Enver T,de Bruijn MFTR

13-0431-82 was used in Immunocytochemistry-immunoflourescence to investigate how and when the onset of circulation affects Hematopoietic stem cells emergence.

Tue Dec 14 00:00:00 UTC 2021

Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA.

Immunity

Soni C,Perez OA,Voss WN,Pucella JN,Serpas L,Mehl J,Ching KL,Goike J,Georgiou G,Ippolito GC,Sisirak V,Reizis B

13-0431-82 was used in Flow Cytometry to establish extrafollicular B cell differentiation into short-lived AFCs as a key mechanism of anti-DNA autoreactivity and reveal a major contribution of pDCs, endosomal Toll-like receptors (TLRs), and IFN-I to this pathway.

Tue Jun 16 00:00:00 UTC 2020

Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing.

Nature communications

Li K,Liu Y,Cao H,Zhang Y,Gu Z,Liu X,Yu A,Kaphle P,Dickerson KE,Ni M,Xu J

Published figure using CD43 monoclonal antibody (Product # 13-0431-82) in Flow Cytometry

Fri Jan 24 00:00:00 UTC 2020

The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates.

Scientific reports

Sun A,Xu K,Liu H,Li H,Shi Y,Zhu X,Liang T,Li X,Cao X,Ji Y,Jiang T,Xu C,Liu X

Published figure using CD43 monoclonal antibody (Product # 13-0431-82) in Flow Cytometry

Thu Mar 05 00:00:00 UTC 2020

53BP1 cooperation with the REV7-shieldin complex underpins DNA structure-specific NHEJ.

Nature

Ghezraoui H,Oliveira C,Becker JR,Bilham K,Moralli D,Anzilotti C,Fischer R,Deobagkar-Lele M,Sanchiz-Calvo M,Fueyo-Marcos E,Bonham S,Kessler BM,Rottenberg S,Cornall RJ,Green CM,Chapman JR

Published figure using CD43 monoclonal antibody (Product # 13-0431-82) in Flow Cytometry

Wed Aug 01 00:00:00 UTC 2018

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD43 monoclonal antibody (Product # 13-0431-82) in Flow Cytometry

Sun Apr 01 00:00:00 UTC 2018

TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK.

The Journal of experimental medicine

Schweighoffer E,Nys J,Vanes L,Smithers N,Tybulewicz VLJ

13043182 was used in flow cytometry to show that TLR4 signals through the BCR leading to activation of SYK, ERK, and AKT as well as through MYD88 leading to activation of NFkappaB

Mon May 01 00:00:00 UTC 2017

TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK.

The Journal of experimental medicine

Schweighoffer E,Nys J,Vanes L,Smithers N,Tybulewicz VLJ

13043182 was used in flow cytometry to show that TLR4 signals through the BCR leading to activation of SYK, ERK, and AKT as well as through MYD88 leading to activation of NFkappaB

Mon May 01 00:00:00 UTC 2017

Understanding Hematopoietic Stem Cell Development through Functional Correlation of Their Proliferative Status with the Intra-aortic Cluster Architecture.

Stem cell reports

Batsivari A,Rybtsov S,Souilhol C,Binagui-Casas A,Hills D,Zhao S,Travers P,Medvinsky A

13-0431 was used in Flow cytometry/Cell sorting to observe time-specific changes in intra-aortic hematopoietic clusters corresponding to HSC maturation stages.

Tue Jun 06 00:00:00 UTC 2017

Function and regulation of self-reactive marginal zone B cells in autoimmune arthritis.

Cellular & molecular immunology

Palm AK,Friedrich HC,Mezger A,Salomonsson M,Myers LK,Kleinau S

13-0431 was used in Flow cytometry/Cell sorting to highlight self-reactive marginal zone B cells as initiators of the autoimmune response in collagen-induced arthritis.

Wed Jul 01 00:00:00 UTC 2015

BAFF activation of the ERK5 MAP kinase pathway regulates B cell survival.

The Journal of experimental medicine

Jacque E,Schweighoffer E,Tybulewicz VL,Ley SC

13-0431 was used in Flow cytometry/Cell sorting to reveal a potentially critical role for the MEK5-ERK5 MAP kinase signalling pathway in BAFF-induced mature B cell survival and homeostatic maintenance of B2 cell numbers.

Mon Jun 01 00:00:00 UTC 2015

The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.

Immunity & ageing : I & A

Matsuda T,Yanase S,Takaoka A,Maruyama M

Published figure using CD43 monoclonal antibody (Product # 13-0431-82) in Flow Cytometry

Mon Mar 02 00:00:00 UTC 2015

Conditional deletion of ferritin h in mice reduces B and T lymphocyte populations.

PloS one

Vanoaica L,Richman L,Jaworski M,Darshan D,Luther SA,Kühn LC

13-0431 was used in Flow cytometry/Cell sorting to investigate the effect of ferritin H deletion on lymphocytes, showing that it reduces B and T lymphocyte populations.

Tue Jan 13 00:00:00 UTC 2015

Minor cell-death defects but reduced tumor latency in mice lacking the BH3-only proteins Bad and Bmf.

Oncogene

Baumgartner F,Woess C,Pedit V,Tzankov A,Labi V,Villunger A

13-0431 was used in Flow cytometry/Cell sorting to show that Bad and Bmf co-regulate lymphocyte homeostasis and limit spontaneous transformation in mice.

Thu Jan 31 00:00:00 UTC 2013

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using CD43 monoclonal antibody (Product # 13-0431-82) in Flow Cytometry

Fri Nov 01 00:00:00 UTC 2013

TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.

PLoS genetics

Gómez-Herreros F,Romero-Granados R,Zeng Z,Alvarez-Quilón A,Quintero C,Ju L,Umans L,Vermeire L,Huylebroeck D,Caldecott KW,Cortés-Ledesma F

Published figure using CD43 monoclonal antibody (Product # 13-0431-82) in Flow Cytometry

Thu Jun 13 00:00:00 UTC 2013

Local renal autoantibody production in lupus nephritis.

Journal of the American Society of Nephrology : JASN

Espeli M,Bökers S,Giannico G,Dickinson HA,Bardsley V,Fogo AB,Smith KG

13-0431 was used in Flow cytometry/Cell sorting to identify the kidney as a major reservoir of autoreactive plasma cells in murine models of lupus nephritis that may contribute significantly to disease progression.

Tue Feb 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: 3E8 antigen; B-cell differentiation antigen LP-3; CD43; Leukocyte sialoglycoprotein; Leukosialin; Ly-48; Lymphocyte antigen 48; Sialophorin; sialophorin gpL115

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Gene Aliases: A630014B01Rik; Cd43; Galgp; Ly-48; Ly48; Spn

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UniProt ID: (Mouse) P15702

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Entrez Gene ID: (Mouse) 20737

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Function(s)

protein binding protein domain specific binding

Process(es)

response to protozoan negative regulation of type IV hypersensitivity positive regulation of protein phosphorylation negative regulation of cell adhesion cell surface receptor signaling pathway T cell costimulation positive regulation of T cell proliferation negative regulation of T cell proliferation positive regulation of tumor necrosis factor biosynthetic process defense response to bacterium negative thymic T cell selection regulation of defense response to virus regulation of immune response negative regulation of T cell activation thymocyte aggregation apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD43 Monoclonal Antibody (eBioR2/60), Biotin, eBioscience™

CD43 Antibody (13-0431-82) in Flow

Product Details

13-0431-82

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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