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CPAMD8 Antibody (PA5-114401) in IHC (P)
Immunohistochemistry analysis of CPAMD8 in paraffin-embedded human mammary cancer tissue. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in CPAMD8 polyclonal antibody (Product # PA5-114401) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) wit... View More
Product Details
PA5-114401
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CPAMD8 recombinant protein (Position: R58-D234).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2884857
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human T-47D whole cell, human U2OS whole cell, human K562 whole cell, human THP-1 whole cell, monkey COS-7 whole cell. IHC: human lung cancer tissue, human mammary cancer tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The CPAMD8 gene, known as C3 and pregnancy zone protein-like alpha-2-macroglobulin domain-containing 8, is involved in the development of ocular tissues. It has been linked to anterior segment dysgenesis (ASD) and congenital glaucoma, indicating its critical role in eye development. CPAMD8 functions primarily within the non-pigmented epithelium and might participate in cellular communication and structural integrity during the differentiation of retinal organoids. Loss-of-function mutations in CPAMD8 disrupt normal eye formation, leading to various ocular disorders. Recent advancements, such as the establishment of CPAMD8-GFP reporter human embryonic stem cell lines, enable real-time monitoring of its expression, thereby facilitating exploration of its biological functions in retinal differentiation processes. Bioinformatic evaluations and CRISPR/Cas9 technology have provided insights into CPAMD8's role in eye development, with gene editing approaches helping delineate its involvement in ocular health and disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-2 macroglobulin family protein VIP; C3 and PZP-like, alpha-2-macroglobulin domain containing 8
Gene Aliases: K-CAP; VIP
Entrez Gene ID: (Human) 27151
Performance Guarantee
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