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Rockland
GAPDH Polyclonal Antibody
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FIGURE: 1 / 1
GAPDH Antibody (600-401-GV0) in WB
Western Blot of Rabbit anti-GAPDH antibody. Lane 1: Opal Pre-stained ladder (p/n MB-210-0500). Lane 2: NIH/3T3 Lysate (p/n W10-000-358). Lane 3: HEK293 lysate (p/n W09-000-365). Lane 4: MOLT-4 Lysate (p/n W09-001-GK2). Lane 5: A549 Lysate (p/n W09-001-372). Lane 6: HeLa Lysate (p/n W09-000-364). Lane 7: NIH 3T3 Nuclear Lysate (W10-001-A74). Lane 8: HeLa Nuclear Lysate (p/n W09-001-367). Lane 9: Jurkat Lysate (p/n W09-001-370). Lane 10: Opal Pre-stained ladder (p/n MB-210-0500). Load: 35 µg per lane. Primary antibody: GAPDH antibody at ... View More
Product Details
600-401-GV0
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
GAPDH Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH).
Conjugate
Form
Liquid
Concentration
1.08 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-GAPDH Antibody is directed against human GAPDH protein. A BLAST analysis was used to suggest that this antibody would react with GAPDH from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aging-associated gene 9 protein; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
Gene Aliases: CDABP0047; G3PD; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; OK/SW-cl.12
UniProt ID: (Human) P04406, (Human) O14556, (Mouse) P16858, (Mouse) Q64467
Entrez Gene ID: (Human) 2597, (Human) 26330, (Mouse) 14433, (Mouse) 14447
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
protein binding
microtubule binding
peptidyl-cysteine S-nitrosylase activity
identical protein binding
NADP binding
NAD binding
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
gluconeogenesis
spermatid development
sperm motility
positive regulation of glycolytic process
canonical glycolysis
glucose metabolic process
glycolytic process
oxidation-reduction process
microtubule cytoskeleton organization
regulation of macroautophagy
negative regulation of translation
peptidyl-cysteine S-trans-nitrosylation
protein stabilization
neuron apoptotic process
cellular response to interferon-gamma
carbohydrate metabolic process
apoptotic process
multicellular organism development
response to ammonium ion
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