GenScript

GFP Polyclonal Antibody, THE™

GFP Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

GFP Antibody (A01704-100) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of GFP fusion gene transfected HEK293 cells. using THE™ GFP Antibody, pAb, Rabbit (GenScript, A01704) and Rabbit IgG Control (Whole Molecule), Purified (GenScript, A01008). The signal was developed with R-PE conjugated Goat Anti-Rabbit IgG.

Product Image Gallery

Product Details

A01704-100

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

Immunocytochemistry (ICC/IF)

1-3 µg/mL

Flow Cytometry (Flow)

1-3 µg 1 x 10^6 cells

Immunoprecipitation (IP)

1-5 µg/mL

Miscellaneous PubMed (MISC)

View 10 publications 10 publications

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

purified recombinant full-length GFP protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y.GenScript THE™ GFP Antibody, pAb, Rabbit is developed in rabbit using purified recombinant full-length GFP protein.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GCNA Interacts with Spartan and Topoisomerase II to Regulate Genome Stability.

Developmental cell

Dokshin GA,Davis GM,Sawle AD,Eldridge MD,Nicholls PK,Gourley TE,Romer KA,Molesworth LW,Tatnell HR,Ozturk AR,de Rooij DG,Hannon GJ,Page DC,Mello CC,Carmell MA

Mon Jan 06 00:00:00 UTC 2020

Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.

The Plant cell

Nagashima Y,Ma Z,Liu X,Qian X,Zhang X,von Schaewen A,Koiwa H

Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-b1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell-wall biosynthesis, and abiotic stress tolerance. After insertion into the endoplasmic reticulum (ER), KOR1 cycles between the trans-Golgi network (TGN) and the plasma membrane (PM). From the TGN, the protein is targeted to growing cell plates during cell division. These processes are governed by mul

Sat Feb 01 00:00:00 UTC 2020

DENN domain-containing protein FAM45A regulates the homeostasis of late/multivesicular endosomes.

Biochimica et biophysica acta. Molecular cell research

Zhang J,Zhang K,Qi L,Hu Q,Shen Z,Liu B,Deng J,Zhang C,Zhang Y

DENN (differentially expressed in normal cells and neoplasia) domain-containing proteins are a family of guanine nucleotide exchange factors (GEFs) for Rab small GTPases and coordinate a plethora of intracellular membrane trafficking events. FAM45A is a non-classical DENN domain protein whose function was unknown. In this study we characterized cellular roles of FAM45A. We found that FAM45A localized mainly in late/multivesicular endosomes. Depletion of FAM45A resulted in clustering of endosomes

Wed May 01 00:00:00 UTC 2019

A Tyrosine Phosphorylation Cycle Regulates Fungal Activation of a Plant Receptor Ser/Thr Kinase.

Cell host & microbe

Liu J,Liu B,Chen S,Gong BQ,Chen L,Zhou Q,Xiong F,Wang M,Feng D,Li JF,Wang HB,Wang J

Plants initiate immunity by cell-surface pattern-recognition receptors (PRRs) which perceive non-self molecules. PRRs are predominantly receptor serine/threonine (Ser/Thr) kinases that are evolutionarily related to animal interleukin-1 receptor-associated kinase (IRAK)/Pelle-soluble kinases. However how the activity of these receptor kinases is modulated remains poorly understood. We report that the Arabidopsis PRR chitin elicitor receptor kinase 1 (CERK1) is autophosphorylated in unstimulated c

Wed Feb 14 00:00:00 UTC 2018

ZNFX-1 Functions within Perinuclear Nuage to Balance Epigenetic Signals.

Molecular cell

Ishidate T,Ozturk AR,Durning DJ,Sharma R,Shen EZ,Chen H,Seth M,Shirayama M,Mello CC

Thu May 17 00:00:00 UTC 2018

The tail domain of the Aspergillus fumigatus class V myosin MyoE orchestrates septal localization and hyphal growth.

Journal of cell science

Renshaw H,Vargas-Muñiz JM,Juvvadi PR,Richards AD,Waitt G,Soderblom EJ,Moseley MA,Steinbach WJ

Myosins are critical motor proteins that contribute to the secretory pathway, polarized growth, and cytokinesis. The globular tail domains of class V myosins have been shown to be important for cargo binding and actin cable organization. Additionally, phosphorylation plays a role in class V myosin cargo choice. Our previous studies on the class V myosin MyoE in the fungal pathogen Aspergillus fumigatus confirmed its requirement for normal morphology and virulence. However, the domains and molecu

Wed Feb 07 00:00:00 UTC 2018

C-terminus Proteolysis and Palmitoylation Cooperate for Optimal Plasma Membrane Localization of RasA in Aspergillus fumigatus.

Frontiers in microbiology

Al Abdallah Q,Martin-Vicente A,Souza ACO,Ge W,Fortwendel JR

RasA is a major regulator of fungal morphogenesis and virulence in . The proper localization of RasA to the plasma membrane is essential for the formation of invasive hyphae during infection. In yeast the localization of Ras2p to the plasma membrane is orchestrated by several post-translational modifications (PTM) at the C-terminal CAAX box that are thought to occur in sequential order. These PTMs include: (1) CAAX motif farnesylation by the farnesyltransferase complex composed of Ram1p and Ram2

Tue Sep 29 00:00:00 UTC 2020

Cartilage Derived from Bone Marrow Mesenchymal Stem Cells Expresses Lubricin In Vitro and In Vivo.

PloS one

Nakagawa Y,Muneta T,Otabe K,Ozeki N,Mizuno M,Udo M,Saito R,Yanagisawa K,Ichinose S,Koga H,Tsuji K,Sekiya I

OBJECTIVE:Lubricin expression in the superficial cartilage will be a crucial factor in the success of cartilage regeneration. Mesenchymal stem cells (MSCs) are an attractive cell source and the use of aggregates of MSCs has some advantages in terms of chondrogenic potential and efficiency of cell adhesion. Lubricin expression in transplanted MSCs has not been fully elucidated so far. Our goals were to determine (1) whether cartilage pellets of human MSCs expressed lubricin in vitro chondrogenesi

Mon Jul 25 00:00:00 UTC 2016

Tissue-specific mitotic bookmarking by hematopoietic transcription factor GATA1.

Cell

Kadauke S,Udugama MI,Pawlicki JM,Achtman JC,Jain DP,Cheng Y,Hardison RC,Blobel GA

SummaryTissue-specific transcription patterns are preserved throughout cell divisions to maintain lineage fidelity. We investigated whether transcription factor GATA1 plays a role in transmitting hematopoietic gene expression programs through mitosis when transcription is transiently silenced. Live-cell imaging revealed that a fraction of GATA1 is retained focally within mitotic chromatin. ChIP-seq of highly purified mitotic cells uncovered that key hematopoietic regulatory genes are occupi

Fri Aug 17 00:00:00 UTC 2012

A novel biotinylated lipid raft reporter for electron microscopic imaging of plasma membrane microdomains.

Journal of lipid research

Krager KJ,Sarkar M,Twait EC,Lill NL,Koland JG

The submicroscopic spatial organization of cell surface receptors and plasma membrane signaling molecules is readily characterized by electron microscopy (EM) via immunogold labeling of plasma membrane sheets. Although various signaling molecules have been seen to segregate within plasma membrane microdomains, the biochemical identity of these microdomains and the factors affecting their formation are largely unknown. Lipid rafts are envisioned as submicron membrane subdomains of liquid ordered

Mon Oct 01 00:00:00 UTC 2012

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more