Invitrogen

IGF1 Polyclonal Antibody, Biotin, PeproTech®

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

500-P11BT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human IGF-I
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929313

Product Specific Information

AA Sequence of recombinant protein: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-I. Anti-Human IGF-I-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human IGF-I by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IGF-I (500-P11) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IGF-I.

Western Blot: To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P11BT-1MG will be provided as 2 x 500 µg

Target Information

IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H-IGF-1; IGF; IGF-I; IGF-IA; IGF-IB; IGF1A; IGFIa; Insulin like growth factor; Insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); Insulin-like growth factor I; insulin-like growth factor IB; M-IGF-1; Mechano growth factor; MGF; OTTHUMP00000195084; R-IGF-1; Somatomedin C; Somatomedin-C

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Gene Aliases: IBP1; IGF-1; IGF-I; IGF1; IGFI; MGF

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UniProt ID: (Human) P05019

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Entrez Gene ID: (Human) 3479

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Function(s)
insulin receptor binding insulin-like growth factor receptor binding integrin binding hormone activity steroid binding protein binding growth factor activity
Process(es)
activation of MAPK activity skeletal system development response to hypoxia cell activation platelet degranulation DNA replication regulation of translation movement of cell or subcellular component signal transduction Ras protein signal transduction muscle organ development female pregnancy aging response to nutrient memory cell proliferation positive regulation of cell proliferation response to heat glycolate metabolic process regulation of gene expression positive regulation of glycoprotein biosynthetic process positive regulation of cardiac muscle hypertrophy phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling response to activity skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration muscle hypertrophy myotube cell development positive regulation of smooth muscle cell migration proteoglycan biosynthetic process positive regulation of cell migration response to caffeine activation of protein kinase B activity response to lipopolysaccharide cellular response to insulin stimulus positive regulation of protein import into nucleus, translocation negative regulation of smooth muscle cell apoptotic process response to nicotine bone mineralization involved in bone maturation regulation of multicellular organism growth positive regulation of activated T cell proliferation positive regulation of tyrosine phosphorylation of Stat5 protein negative regulation of apoptotic process positive regulation of DNA binding positive regulation of MAPK cascade protein kinase B signaling positive regulation of insulin-like growth factor receptor signaling pathway cellular protein metabolic process myoblast differentiation positive regulation of fat cell differentiation positive regulation of osteoblast differentiation positive regulation of glycogen biosynthetic process positive regulation of DNA replication positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of Ras protein signal transduction insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of protein secretion positive regulation of peptidyl-tyrosine phosphorylation protein stabilization detection of mechanical stimulus involved in sensory perception response to glucocorticoid myoblast proliferation regulation of calcium ion transport negative regulation of oocyte development positive regulation of cell growth involved in cardiac muscle cell development ERK1 and ERK2 cascade positive regulation of calcineurin-NFAT signaling cascade cellular response to electrical stimulus cellular response to estradiol stimulus negative regulation of release of cytochrome c from mitochondria negative regulation of neuron death positive regulation of trophectodermal cell proliferation negative regulation of cholangiocyte apoptotic process positive regulation of transcription regulatory region DNA binding negative regulation of extrinsic apoptotic signaling pathway
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