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IL-1 beta Antibody (PA5-47204) in Neu
Neutralization of IL-1 beta in D10.G4.1 mouse helper T cell line. Samples were incubated in IL-1 beta polyclonal antibody (Product # PA5-47204) using a dilution of 50 pg/mL. Recombinant Feline IL‚1β/IL‚1F2 stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‚1β/IL‚1F2 is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL‚1β/IL‚1F2 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.02‚0.08 µg/m... View More
Product Details
PA5-47204
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant feline IL-1 beta/IL-1F2 Ala116-Ser267
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608803
Product Specific Information
In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human IL-1 beta, recombinant mouse IL-1 beta, and recombinant canine IL-1 beta is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1 beta; Interleukin-1b; interleukin-beta; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta
Gene Aliases: IL1B
UniProt ID: (Cat) P41687
Entrez Gene ID: (Cat) 768274
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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