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          • Primary Antibodies ›
          • IL-15 Antibodies

          Invitrogen

          IL-15 Monoclonal Antibody (ct2nu), Functional Grade, eBioscience™

          4 References
          View all (74) IL-15 antibodies

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          Cite IL-15 Monoclonal Antibody (ct2nu), Functional Grade, eBioscience™

          • Antibody Testing Data (1)
          IL-15 Antibody in Functional Assay (Functional)
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          IL-15 Antibody in Functional Assay (Functional)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          IL-15 Antibody (16-0157-82) in Functional

          Neutralization of Human IL-15 Recombinant Protein activity, as measured by CTLL-2 proliferation. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          IL-15 Antibody in Functional Assay (Functional)

          Product Details

          16-0157-82

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          Neutralization (Neu)

          Assay-Dependent
          -

          Functional Assay (Functional)

          Assay-Dependent
          View 1 publication 1 publication

          Inhibition Assays (IA)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          ct2nu

          Immunogen

          Recombinant Human IL-15

          Conjugate

          Functional Grade Functional Grade Functional Grade

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10596500

          Product Specific Information

          Description: The ct2nu monoclonal antibody reacts with human IL-15 and neutralizes its bioactivity. IL-15, or IL-T, is a 14 kDa proinflammatory cytokine and member of the IL-2 family. It plays a role in the development and maturation of macrophages and dendritic cells, and is essential to the proliferation and survival of NK cells and CD8^+ T cells.

          IL-15 is expressed by antigen-presenting cells, epithelial cells, and stromal cells. IL-15 activates the Jak/STAT pathway through its heterotrimeric receptor. Two subunits of this receptor, the beta and gamma chains, are shared with the rest of the family, while the alpha subunit is unique to IL-15. In mice, the alpha chain is required to chaperone IL-15 from the ER to secretion. This chaperoning is not necessary in humans, and free IL-15 is detectable in human biological samples. However, the receptor-cytokine complex has been indentified in humans and demonstrates agonistic activity several-fold higher than IL-15 alone.

          Applications Reported: The monoclonal antibody ct2nu reacts with and inhibits the bioactivity of human IL-15.

          Applications Tested: The ND50 of this antibody, as measured by inhibition of proliferation in CTLL-2 cells, is 1-10 ng/mL in the presence of 0.5 ng/mL recombinant human IL-15. Neutralization dose will vary based on cell type, cytokine concentration, and assay method. This antibody should be carefully titrated for optimal performance in the assay of interest.

          Storage and handling: Use in a sterile environment.

          Filtration: 0.2 µm post-manufacturing filtered.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

          Aggregation: Less than 10%, as determined by HPLC.

          Target Information

          IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: il 15; IL-15; ILN; Interleukin; Interleukin-15; Interleukin15; M-IL-15; MGC9721

          View more View less

          Gene Aliases: IL-15; IL15

          View more View less

          UniProt ID: (Human) P40933

          View more View less

          Entrez Gene ID: (Human) 3600

          View more View less

          Function(s)
          cytokine activity cytokine receptor binding protein binding
          Process(es)
          natural killer cell differentiation NK T cell proliferation inflammatory response immune response signal transduction cell-cell signaling aging positive regulation of cell proliferation skeletal muscle atrophy hyaluronan metabolic process positive regulation of interleukin-17 production positive regulation of natural killer cell proliferation positive regulation of natural killer cell differentiation positive regulation of tissue remodeling positive regulation of T cell proliferation tyrosine phosphorylation of Stat5 protein positive regulation of tyrosine phosphorylation of Stat3 protein extrathymic T cell selection regulation of T cell differentiation cell maturation lymph node development negative regulation of smooth muscle cell proliferation regulation of defense response to virus by host positive regulation of inflammatory response positive regulation of immune response cellular response to vitamin D positive regulation of protein O-linked glycosylation
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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