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Invitrogen
IL-2 Polyclonal Antibody, Biotin, eBioscience™
Product Details
13-7028-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_466900
Product Specific Information
Description: The rabbit anti-human IL-2 polyclonal antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.
Applications Reported: This polyclonal antibody has been reported for use in detection of human IL-2 by ELISA.
Applications Tested: The biotinylated polyclonal rabbit anti-human IL-2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-2 in combination with the purified MQ1-17H12 (14-7029) antibody for capture and recombinant human IL-2 (14-8029) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: aldesleukin; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Aliases: IL-2; IL2; lymphokine; TCGF
UniProt ID: (Human) P60568
Entrez Gene ID: (Human) 3558
MAPK cascade
negative regulation of protein phosphorylation
adaptive immune response
negative regulation of B cell apoptotic process
immune response
cell adhesion
positive regulation of cytosolic calcium ion concentration
protein kinase C-activating G-protein coupled receptor signaling pathway
cell-cell signaling
positive regulation of cell proliferation
natural killer cell activation
T cell differentiation
positive regulation of cell growth
positive regulation of B cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of tissue remodeling
positive regulation of activated T cell proliferation
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of apoptotic process
positive regulation of GTPase activity
response to ethanol
positive regulation of regulatory T cell differentiation
negative regulation of heart contraction
positive regulation of transcription from RNA polymerase II promoter
regulation of T cell homeostatic proliferation
positive regulation of isotype switching to IgG isotypes
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
positive regulation of inflammatory response
positive regulation of immunoglobulin secretion
positive regulation of dendritic spine development
extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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