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IL-2 Antibody (202-401-B95) in WB
Western blot using Rocklands protein-A purified anti-Cat IL-2 antibody shows detection of recombinant Cat IL-2 at 15.4 kDa, raised in yeast. Primary antibody was diluted to 1 µg/ml. 3% BSA from Rocklands BSA-30 (Bovine Serum Albumin Solution) was used for blocking. Secondary antibody 611-131-122 (Goat anti-Rabbit IgG IRDye® 800) was used at 1:20,000.
Product Details
202-401-B95
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 134 amino acids of mature cat IL-2.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for cat IL-2 protein. A BLAST analysis was used to suggest cross-reactivity with IL-2 from cat based on 100% homology; cross-reactivity with Northern elephant seal and gray seal based on 90% homology with the immunizing sequence. Cross-reactivity with IL-2 from other sources has not been determined.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Aliases: IL2
UniProt ID: (Cat) Q07885
Entrez Gene ID: (Cat) 751114
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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