Invitrogen

IL-2 Monoclonal Antibody (JES6-5H4), Functional Grade, eBioscience™

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IL-2 Antibody (16-7021-025) in ELISA

Nature communications 2017 - Figure 3 CIC negatively regulates T-cell activation. ( a , b ) Increased CIC expression in activated CD4 + T cells. Western blot analysis for CIC levels in CD4 + T cells during T-cell activation by alpha-CD3/CD28 antibodies ( a ) and in sorted naive (CD44 lo CD62L hi ) and effector/memory (CD44 hi CD62L lo ) CD4 + T cells ( b ). ( c ) ELISA of IL-2. WT and Cic -deficient (cKO) CD4 + CD25 - CD44 lo CD62L hi ... View More

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IL-2 Antibody — Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IL-2 clone JES6-5H4 stains a large subset of CD4+ T cells and a minor subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IL-2 (clone JES6-5H4). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis. {TM}

Product Details

16-7021-025

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 21 publications 21 publications

ELISA (ELISA)

0.5-2 µg/mL

View 5 publications 5 publications

Functional Assay (Functional)

Assay-Dependent

In vitro Assay (IV)

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES6-5H4

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4°C

Shipping conditions

Wet ice

AB_2866200

Product Specific Information

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported: The functional grade purified JES6-5H4 has been reported for use in blocking of fluorochrome conjugated JES6-5H4 in intracellular flow cytometry experiments to serve as specificity control. JES6-5H4 is reported for detection of mouse IL-2 by ELISA, immunoprecipitation, and for neutralizing IL-2 binding and biological activity.

Applications Tested: The JES6-5H4 antibody has been tested by sandwich ELISA for detection of mouse IL-2 in combination with JES6-1A12 and can be used between 0.5-2 µg/mL.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection.

International journal of molecular sciences

Yang G,Wang J,Wang S,Zhu Y

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Sat Aug 20 00:00:00 UTC 2022

Z. morio Hemolymph Relieves E. coli-Induced Mastitis by Inhibiting Inflammatory Response and Repairing the Blood-Milk Barrier.

International journal of molecular sciences

Zou Y,Wang X,Xu J,Wang S,Li S,Zhu Y,Wang J

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Mon Oct 31 00:00:00 UTC 2022

In vivo genome-wide CRISPR screening identifies ZNF24 as a negative NF-κB modulator in lung cancer.

Cell & bioscience

Liu L,Lei Y,Chen W,Zhou Q,Zheng Z,Zeng G,Liu W,Feng P,Zhang Z,Yu L,Chen L

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Thu Dec 01 00:00:00 UTC 2022

Impaired Treg-DC interactions contribute to autoimmunity in leukocyte adhesion deficiency type 1.

JCI insight

Klaus T,Wilson AS,Vicari E,Hadaschik E,Klein M,Helbich SSC,Kamenjarin N,Hodapp K,Schunke J,Haist M,Butsch F,Probst HC,Enk AH,Mahnke K,Waisman A,Bednarczyk M,Bros M,Bopp T,Grabbe S

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Thu Dec 22 00:00:00 UTC 2022

A Methyltransferase-Defective Vesicular Stomatitis Virus-Based SARS-CoV-2 Vaccine Candidate Provides Complete Protection against SARS-CoV-2 Infection in Hamsters.

Journal of virology

Lu M,Zhang Y,Dravid P,Li A,Zeng C,Kc M,Trivedi S,Sharma H,Chaiwatpongsakorn S,Zani A,Kenney A,Cai C,Ye C,Liang X,Qiu J,Martinez-Sobrido L,Yount JS,Boyaka PN,Liu SL,Peeples ME,Kapoor A,Li J

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Mon Sep 27 00:00:00 UTC 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Mon May 10 00:00:00 UTC 2021

Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors.

EMBO molecular medicine

Fan Z,Tian Y,Chen Z,Liu L,Zhou Q,He J,Coleman J,Dong C,Li N,Huang J,Xu C,Zhang Z,Gao S,Zhou P,Ding K,Chen L

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Mon Jun 08 00:00:00 UTC 2020

Deletion Timing of Cic Alleles during Hematopoiesis Determines the Degree of Peripheral CD4⁺ T Cell Activation and Proliferation.

Immune network

Park GY,Lee GW,Kim S,Hong H,Park JS,Cho JH,Lee Y

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in ELISA

Thu Oct 01 00:00:00 UTC 2020

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Thu Nov 05 00:00:00 UTC 2020

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model.

Vaccines

Reeder SM,Reuschel EL,Bah MA,Yun K,Tursi NJ,Kim KY,Chu J,Zaidi FI,Yilmaz I,Hart RJ,Perrin B,Xu Z,Humeau L,Weiner DB,Aly ASI

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Fri Jan 10 00:00:00 UTC 2020

Oxymatrine and Cisplatin Synergistically Enhance Anti-tumor Immunity of CD8⁺ T Cells in Non-small Cell Lung Cancer.

Frontiers in oncology

Ye J,Zou MM,Li P,Lin XJ,Jiang QW,Yang Y,Huang JR,Yuan ML,Xing ZH,Wei MN,Li Y,Shi Z,Liu H

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Thu Oct 01 00:00:00 UTC 2020

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2017

Vaccination with High-Affinity Epitopes Impairs Antitumor Efficacy by Increasing PD-1 Expression on CD8⁺ T Cells.

Cancer immunology research

Zahm CD,Colluru VT,McNeel DG

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Tue Aug 01 00:00:00 UTC 2017

A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2⁺ T Cells.

Frontiers in immunology

Yu Q,Wang X,Fan X

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Wed Nov 20 00:00:00 UTC 2019

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

EBioMedicine

Ma J,Teng X,Wang X,Fan X,Wu Y,Tian M,Zhou Z,Li L

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Tue Aug 01 00:00:00 UTC 2017

Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5.

Nature communications

Park S,Lee S,Lee CG,Park GY,Hong H,Lee JS,Kim YM,Lee SB,Hwang D,Choi YS,Fryer JD,Im SH,Lee SW,Lee Y

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in ELISA

Wed Jul 12 00:00:00 UTC 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Wed Oct 11 00:00:00 UTC 2017

miR-150 regulates obesity-associated insulin resistance by controlling B cell functions.

Scientific reports

Ying W,Tseng A,Chang RC,Wang H,Lin YL,Kanameni S,Brehm T,Morin A,Jones B,Splawn T,Criscitiello M,Golding MC,Bazer FW,Safe S,Zhou B

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2016

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Fri Mar 11 00:00:00 UTC 2016

mTOR kinase inhibitors promote antibody class switching via mTORC2 inhibition.

Proceedings of the National Academy of Sciences of the United States of America

Limon JJ,So L,Jellbauer S,Chiu H,Corado J,Sykes SM,Raffatellu M,Fruman DA

16-7021 was used in in vitro experiments to further elucidate the complexity of mTOR signalling in B cells.

Tue Nov 25 00:00:00 UTC 2014

TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.

European journal of immunology

Reba SM,Li Q,Onwuzulike S,Ding X,Karim AF,Hernandez Y,Fulton SA,Harding CV,Lancioni CL,Nagy N,Rodriguez ME,Wearsch PA,Rojas RE

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Thu May 01 00:00:00 UTC 2014

Analysis of the vaccine potential of plasmid DNA encoding nine mycolactone polyketide synthase domains in Mycobacterium ulcerans infected mice.

PLoS neglected tropical diseases

Roupie V,Pidot SJ,Einarsdottir T,Van Den Poel C,Jurion F,Stinear TP,Huygen K

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in ELISA

Mon Sep 08 00:00:00 UTC 2014

Sterol regulatory element-binding proteins are essential for the metabolic programming of effector T cells and adaptive immunity.

Nature immunology

Kidani Y,Elsaesser H,Hock MB,Vergnes L,Williams KJ,Argus JP,Marbois BN,Komisopoulou E,Wilson EB,Osborne TF,Graeber TG,Reue K,Brooks DG,Bensinger SJ

16-7021 was used in Flow cytometry/Cell sorting to provide insights into the molecular signals that underlie the metabolic reprogramming of CD8(+) T cells during the transition from quiescence to activation.

Wed May 01 00:00:00 UTC 2013

Environmental effects on protection against Mycobacterium tuberculosis after immunization with Ad85A.

Vaccine

Beverley P,Ronan E,Lee L,Arnold I,Bolinger B,Powrie F,Tchilian E

16-7021 was used in Flow cytometry/Cell sorting to show that exposure to environmental microorganisms can alter the protective immune response to a parenterally administered subunit vaccine.

Mon Feb 04 00:00:00 UTC 2013

Enhanced efficacy and reduced toxicity of multifactorial adjuvants compared with unitary adjuvants as cancer vaccines.

Blood

Ahonen CL,Wasiuk A,Fuse S,Turk MJ,Ernstoff MS,Suriawinata AA,Gorham JD,Kedl RM,Usherwood EJ,Noelle RJ

16-7021 was used in Flow cytometry/Cell sorting to report the therapeutic effects of combinatorial use of CD40 and TLR agonists as a cancer vaccine.

Sat Mar 15 00:00:00 UTC 2008

Modulation of pulmonary dendritic cell function during mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Liu Y,Harding CV,Boom WH

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in ELISA

Fri Feb 01 00:00:00 UTC 2008

Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Harding CV,Boom WH

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in ELISA

Tue May 01 00:00:00 UTC 2007

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IL-2 Monoclonal Antibody (JES6-5H4), Functional Grade, eBioscience™

IL-2 Antibody (16-7021-025) in ELISA

Product Details

16-7021-025

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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