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Product Details
MA1-82421
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Ferret,
Mustelid,
Mink,
Sheep,
Pig,
Rabbit
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
8M6
Immunogen
Recombinant ovine IL-8
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS
Contains
<0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2126346
Product Specific Information
This antibody has also been reported to neutralize the bioactivity of ovine IL-8, however prior removal of sodium azide may be necessary for functional assays. Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Ovine Interleukin-8 antibody, clone 8M6 recognizes ovine interleukin-8 (IL-8), also known as C-X-C motif chemokine 8.
Target Information
Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; AMCF-I; ANX7; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; IL-8; ILN; Interleukin; interleukin 8; Interleukin-8; Interleukin8; NAP-1; neutrophil activating peptide 1; Neutrophil attractant/activation protein 1; neutrophil attractant/activation protein-1; Permeability factor 1; PF1; RP11-537A6.8; RPF1; SNX; SYNEXIN
Gene Aliases: AMCF-I; BOS_6776; CXCL8; IL-8; IL8
UniProt ID: (Dog) P41324, (Sheep) P36925, (Bovine) P79255, (Pig) P26894, (Rabbit) P19874
Entrez Gene ID: (Dog) 403850, (Sheep) 443418, (Bovine) 280828, (Pig) 396880, (Rabbit) 100009129
inflammatory response
immune response
cell cycle arrest
G-protein coupled receptor signaling pathway
regulation of cell adhesion
neutrophil chemotaxis
receptor internalization
response to lipopolysaccharide
response to endoplasmic reticulum stress
intracellular signal transduction
neutrophil activation
regulation of cell proliferation
cellular response to fibroblast growth factor stimulus
regulation of single stranded viral RNA replication via double stranded DNA intermediate
negative regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
embryonic digestive tract development
induction of positive chemotaxis
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to interleukin-1
cellular response to tumor necrosis factor
positive regulation of neutrophil chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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