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IL18BP Antibody (500-P153G-1MG) in ELISA
Sandwich ELISA: To detect Murine IL-18BP by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL18BP Polyclonal Antibody (Product # 500-P153G-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL18BP Polyclonal Antibody, Biotin (Product # 500-P153GBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-18BP.
Product Details
500-P153G-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IL-18BP
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929510
Product Specific Information
Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine IL-18BP. Anti-Murine IL-18BP-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-18BP matrix.
Sandwich ELISA: To detect Murine IL-18BP by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-18BP (500-P153GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-18BP.
Western Blot: To detect Murine IL-18BP by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-18BP is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Interleukin-18 binding protein (IL-18BP) is a constitutively secreted glycoprotein that acts as a natural antagonist for interleukin-18 (IL-18) by preventing interaction between IL-18 and its receptors. Through this binding, IL-18BP neutralizes the proinflammatory role of IL-18 in cell-mediated immune responses, resulting in reduced IFN-gamma production by T helper type I cells and inhibition of allospecific cytotoxic T lymphocyte (CTL) activity through augmented natural killer (NK) cell cytotoxicity. As an inhibitor of IL-18 and an essential component of skin homeostasis, IL-18BP has been linked to a variety of diseases, including eczema, asthma, and the autoimmune condition, Crohn's disease. The upregulation of IL-18BP by keratinocytes expressing the human papillomavirus (HPV) E7 oncoprotein has been shown to decrease IL-18-induced IFN-gamma production and IL-18-mediated T cell activation. Elevated levels of IL-18 and IL-18BP, as well as proinflammatory cytokines IFN-gamma, TNF-alpha, IL-1beta and IL-8, have been linked to chronic inflammation and observed in macrophages (including Kupffer cells) isolated from lesions and intestinal tissues of Crohn's disease patients. As a member of the immunoglobulin-like class of receptors, IL-18BP contains a single immunoglobulin (Ig) domain; although two (b and d) of the four identified human isoforms (a-d) contain incomplete domains that greatly reduce their binding affinity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: IL-18BP; interferon gamma inducing factor binding protein; Interferon gamma-inducing factor-binding protein; Interleukin-18-binding protein
Gene Aliases: Igifbp; IL-18BP; Il18bp; MC54L
UniProt ID: (Mouse) Q9Z0M9
Entrez Gene ID: (Mouse) 16068
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