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          • Primary Antibodies ›
          • IL-6 Antibodies

          Abnova

          IL6 Monoclonal Antibody (3E8)

          View all (211) IL-6 antibodies

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          Cite IL6 Monoclonal Antibody (3E8)

          • Antibody Testing Data (1)
          IL6 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          IL6 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          IL6 Antibody (H00003569-M05) in WB

          Western Blot analysis of IL6 expression in transfected 293T cell line by IL6 monoclonal antibody (M05), clone 3E8.

          Lane 1: IL6 transfected lysate(23.7 KDa).
          Lane 2: Non-transfected lysate.
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          IL6 Antibody in Western Blot (WB)

          Product Details

          H00003569-M05

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-5 µg/mL
          -

          ELISA (ELISA)

          1-5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3E8

          Immunogen

          IL6 (NP_000591, 29 a.a.-212 a.a.) full-length recombinant protein.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          See Label

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          no preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Sequence of this protein is as follows: SPVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQM

          Target Information

          IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

          View more View less

          Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

          View more View less

          UniProt ID: (Human) P05231

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          Entrez Gene ID: (Human) 3569

          View more View less

          Function(s)
          cytokine activity interleukin-6 receptor binding protein binding growth factor activity
          Process(es)
          neutrophil apoptotic process response to yeast hepatic immune response neutrophil mediated immunity monocyte chemotaxis positive regulation of acute inflammatory response positive regulation of leukocyte chemotaxis negative regulation of protein kinase activity acute-phase response inflammatory response humoral immune response aging positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape response to heat response to cold regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of lipid storage response to auditory stimulus cell growth cytokine-mediated signaling pathway platelet activation response to caffeine endocrine pancreas development neuron projection development response to nutrient levels response to peptidoglycan positive regulation of chemokine production positive regulation of interleukin-6 production response to insulin negative regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation positive regulation of protein import into nucleus, translocation cellular response to hepatocyte growth factor stimulus positive regulation of T cell proliferation response to drug positive regulation of tyrosine phosphorylation of Stat3 protein glucose homeostasis defense response to protozoan positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process response to amino acid positive regulation of MAPK cascade negative regulation of chemokine biosynthetic process regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of nitric oxide biosynthetic process cell redox homeostasis negative regulation of fat cell differentiation positive regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation positive regulation of osteoblast differentiation negative regulation of gluconeogenesis positive regulation of translation positive regulation of DNA replication regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade response to antibiotic muscle cell cellular homeostasis bone remodeling negative regulation of hormone secretion negative regulation of muscle organ development positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation negative regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium defense response to Gram-positive bacterium positive regulation of B cell activation positive regulation of immunoglobulin secretion positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity response to glucocorticoid response to calcium ion response to electrical stimulus defense response to virus positive regulation of protein kinase B signaling positive regulation of transmission of nerve impulse branching involved in salivary gland morphogenesis epithelial cell proliferation involved in salivary gland morphogenesis glucagon secretion interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to dexamethasone stimulus positive regulation of cell proliferation in bone marrow T-helper 17 cell lineage commitment negative regulation of neuron death positive regulation of STAT protein import into nucleus positive regulation of type B pancreatic cell apoptotic process
          It has to be done as per old AB suggested Products section.
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