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Rockland
Oct-4 Polyclonal Antibody
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FIGURE: 1 / 1
Oct-4 Antibody (600-401-A95) in WB
Western blot using Rocklands affinity purified anti-Oct4 antibody shows detection of endogenous Oct4 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2). The band at ~39 kDa (arrow head) corresponds to Oct4. After transfer, the membrane was blocked with 5% BSA. Primary antibody was used at a 1:1,000 dilution in PBS containing 5% BSA. The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with Oct4 (lanes 3 and 4, respectively).
Product Details
600-401-A95
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Horse,
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Oct-4 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with human and mouse Oct-4 protein. Cross-reactivity with Oct-4 from other species has not been tested. A BLAST analysis was used to suggest additional cross-reactivity with Oct-4 from chimpanzee, macaque and horse based on a 100% homology with the immunizing sequence; and mink, dog, bovine and pig based on a 93% homology.
Target Information
POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2
Gene Aliases: Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4; POU5F1
UniProt ID: (Human) Q01860
Entrez Gene ID: (Human) 5460
transcription regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
ubiquitin protein ligase binding
miRNA binding
sequence-specific DNA binding
poly(A) RNA binding
negative regulation of transcription from RNA polymerase II promoter
endodermal cell fate specification
blastocyst development
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
response to wounding
anatomical structure morphogenesis
regulation of asymmetric cell division
regulation of gene expression
somatic stem cell population maintenance
mRNA transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
negative regulation of gene silencing by miRNA
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