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PAI1 Antibody (500-P260-1MG) in ELISA
Sandwich ELISA: To detect Human PAI-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of PAI1 Polyclonal Antibody (Product # 500-P260-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech PAI1 Polyclonal Antibody, Biotin (Product # 500-P260BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PAI-1.
Product Details
500-P260-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human PAI-1
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930126
Product Specific Information
AA Sequence of recombinant protein: VHHPPSYVAH LASDFGVRVF QQVAQASKDR NVVFSPYGVA SVLAMLQLTT GGETQQQIQA AMGFKIDDKG MAPALRHLYK ELMGPWNKDE ISTTDAIFVQ RDLKLVQGFM PHFFRLFRST VKQVDFSEVE RARFIINDWV KTHTKGMISN LLGKGAVDQL TRLVLVNALY FNGQWKTPFP DSSTHRRLFH KSDGSTVSVP MMAQTNKFNY TEFTTPDGHY YDILELPYHG DTLSMFIAAP YEKEVPLSAL TNILSAQLIS HWKGNMTRLP RLLVLPKFSL ETEVDLRKPL ENLGMTDMFR QFQADFTSLS DQEPLHVAQA LQKVKIEVNE SGTVASSSTA VIVSARMAPE EIIMDRPFLF VVRHNPTGTV LFMGQVMEP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PAI-1. Anti-Human PAI-1-specific antibody was purified by affinity chromatography employing an immobilized Human PAI-1 matrix.
Sandwich ELISA: To detect Human PAI-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PAI-1 (500-P260Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PAI-1.
Western Blot: To detect Human PAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PAI-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
PAI1 (plasminogen activator inhibitor 1) belongs to serine protease inhibitor superfamily, and is the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA). Platelets are the main source of the circulating PAI11, but it is synthesized and secreted by many tissue and cell types, including fibroblasts, smooth muscle cells, endothelial cells, hepatocytes, and inflammatory cells. Expression of PAI11 can be regulated at the transcriptional level by many factors including growth factors, cytokines, hormones, inflammatory factors, glucose or lipid metabolites, vascular tone regulating factors, chemicals, and other environmental or physical factors. PAI1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity, thrombophilia and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding. PAI1 mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator, and may promote neuronal survival. Other defects in PAI1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency). Alternatively spliced transcript variants encoding different isoforms of PAI1 have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Endothelial plasminogen activator inhibitor; PAI; plas; Plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; SERPINE-1
Gene Aliases: PAI; PAI-1; PAI1; PLANH1; SERPINE1
UniProt ID: (Human) P05121
Entrez Gene ID: (Human) 5054
chronological cell aging
angiogenesis
platelet degranulation
circadian rhythm
regulation of receptor activity
negative regulation of plasminogen activation
negative regulation of endopeptidase activity
negative regulation of smooth muscle cell migration
positive regulation of blood coagulation
negative regulation of blood coagulation
extracellular matrix organization
negative regulation of cell migration
positive regulation of interleukin-8 production
negative regulation of cell adhesion mediated by integrin
positive regulation of leukotriene production involved in inflammatory response
fibrinolysis
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
positive regulation of receptor-mediated endocytosis
positive regulation of inflammatory response
defense response to Gram-negative bacterium
negative regulation of fibrinolysis
negative regulation of vascular wound healing
negative regulation of wound healing
cellular response to lipopolysaccharide
positive regulation of monocyte chemotaxis
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of smooth muscle cell-matrix adhesion
negative regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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