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Invitrogen
Pepsinogen I Recombinant Rabbit Monoclonal Antibody (HL2137)
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FIGURE: 1 / 5
Pepsinogen I Antibody (MA5-50395) in ELISA
Indirect ELISA analysis performed by coating plate with recombinant full-length NS0 cells expressed, full-length human pepsinogen A protein (97.56-1.52 nM). Coated protein was probed with Pepsinogen I Recombinant Monoclonal Antibody (Product # MA5-50395) at a dilution of 1 μg/mL. An HRP conjugated Goat anti-rabbit IgG antibody was used at a dilution of 1:10,000 to detect bound primary antibody.
Product Details
MA5-50395
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HL2137
Immunogen
Recombinant fragmemt of human Pepsinogen I.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3093703
Product Specific Information
MA5-50395 has sucessfully been used in Indirect ELISA.
Target Information
PGA5: Pepsinogen 5, group I (pepsinogen A). Pepsinogens are the inactive precursors of pepsin, the major acid protease found in the stomach. Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. PGA5 is a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates. PGA5 is inhibited by ovUS-1, a uterine serpin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pepsin A; Pepsin A-5; Pepsin F; pepsinogen 5, group I; pepsinogen 5, group I (pepsinogen A); pepsinogen A5; pepsinogen F; pepsinogen F protein; Pepsinogen-5
Gene Aliases: 1110035E17Rik; Pepf; Pg5; PGA5
UniProt ID: (Human) P0DJD9, (Mouse) Q9D106
Entrez Gene ID: (Human) 5222, (Mouse) 58803, (Rat) 60372
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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