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Invitrogen
Phospho-Estrogen Receptor alpha (Ser118) Recombinant Rabbit Monoclonal Antibody (0X8T4)
FIGURE: 1 / 1
Phospho-Estrogen Receptor alpha (Ser118) Antibody (MA5-35857) in WB
Western blot analysis of Phospho-Estrogen Receptor alpha (Ser118) in extracts of NIH/3T3 cells. Samples were incubated with Phospho-Estrogen Receptor alpha (Ser118) Monoclonal antibody (Product # MA5-35857) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 cells were treated by EGF (100 ng/mL) at 37°C for 30 minutes after serum-star... View More
Product Details
MA5-35857
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
0X8T4
Immunogen
A synthetic phosphorylated peptide around S118 of human Estrogen Receptor alpha (ESR1) (P03372)
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849755
Product Specific Information
Immunogen sequence: QLSPF
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686N23123; ER; ER-alpha; ER36; esr 1; esr-1; Estradiol receptor; Estrogen receptor; hER-alpha36; Nuclear receptor subfamily 3 group A member 1; RP1-130E4.1
Gene Aliases: ER; ER-alpha; ERa; ERalpha; ESR; Esr1; Estr; Estra; Nr3a1
UniProt ID: (Mouse) P19785
Entrez Gene ID: (Mouse) 13982
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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