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Resistin Antibody (500-P182GBT-25UG) in ELISA
Sandwich ELISA: To detect mResistin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Resistin Polyclonal Antibody, Biotin (Product # 500-P182GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Resistin Polyclonal Antibody (Product # 500-P182G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mResistin.
Product Details
500-P182GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 20.2 kDa Recombinant Murine Resistin
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929687
Product Specific Information
AA Sequence of recombinant protein: SSMPLCPIDE AIDKKIKQDF NSLFPNAIKN IGLNCWTVSS RGKLASCPEG TAVLSCSCGS ACGSWDIREE KVCHCQCARI DWTAARCCKL QVAS.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine Resistin. Anti-Murine Resistin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mResistin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine Resistin (500-P182G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mResistin.
Western Blot: To detect Murine Resistin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine Resistin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P182GBT-1MG will be provided as 2 x 500 µg
Target Information
Adipose tissue is an energy reserve in animals and is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice. Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adipose tissue specific secretory factor; Adipose tissue-specific secretory factor; Adipose-specific cysteine-rich secreted protein A12-alpha; ADSF; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1; cysteine-r; Cysteine-rich secreted protein FIZZ3; dominant inhibitory adipocyte-specific secretory factor; dri; found in inflammatory zone 3; MGC126603; MGC126609; Resistin; RSN
Gene Aliases: ADSF; Fizz3; Retn; Rstn; Xcp4
UniProt ID: (Mouse) Q99P87
Entrez Gene ID: (Mouse) 57264
positive regulation of collagen metabolic process
positive regulation of smooth muscle cell migration
response to insulin
fat cell differentiation
positive regulation of smooth muscle cell proliferation
positive regulation of synaptic transmission
negative regulation of feeding behavior
positive regulation of progesterone secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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