Invitrogen

STAT3 Monoclonal Antibody (ST3-5G7)

STAT3 Antibody in Immunocytochemistry (ICC/IF)

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STAT3 Antibody (13-7000) in ICC/IF

Immunofluorescence analysis of STAT3 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with STAT3 Mouse Monoclonal Antibody (Product # 13-7000) at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2817... View More

STAT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

STAT3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with STAT3 siRNA and decrease in signal intensity was observed in western blot application using anti-STAT3 siRNA (Product # 137000). {KD}

Product Details

13-7000

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:500

Immunocytochemistry (ICC/IF)

1:250

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

ChIP assay (ChIP)

Assay-dependent

View 3 publications 3 publications

ChIP-sequencing (ChIP-Seq)

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

View 4 publications 4 publications

Miscellaneous PubMed (MISC)

View 5 publications 5 publications

Product Specifications

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ST3-5G7

Immunogen

A GST-fusion protein containing the unique carboxy terminal domain of the murine STAT3 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

AB_2533031

Target Information

STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Autophagy Quantification and STAT3 Expression in a Human Skin Organ Culture Model for Innate Immunity to Herpes Zoster.

Frontiers in microbiology

Buckingham EM,Girsch J,Jackson W,Cohen JI,Grose C

13-7000 was used in Immunohistochemistry-immunofluorescence to document the autophagy response in human neonatal skin organ culture after infection with varicella-zoster virus.

Mon Apr 08 00:00:00 UTC 2024

Opposing roles of STAT1 and STAT3 in IL-21 function in CD4+ T cells.

Proceedings of the National Academy of Sciences of the United States of America

Wan CK,Andraski AB,Spolski R,Li P,Kazemian M,Oh J,Samsel L,Swanson PA,McGavern DB,Sampaio EP,Freeman AF,Milner JD,Holland SM,Leonard WJ

Published figure using STAT3 monoclonal antibody (Product # 13-7000) in Western Blot

Tue Jul 28 00:00:00 UTC 2015

Opposing roles of STAT1 and STAT3 in IL-21 function in CD4+ T cells.

Proceedings of the National Academy of Sciences of the United States of America

Wan CK,Andraski AB,Spolski R,Li P,Kazemian M,Oh J,Samsel L,Swanson PA,McGavern DB,Sampaio EP,Freeman AF,Milner JD,Holland SM,Leonard WJ

Published figure using STAT3 monoclonal antibody (Product # 13-7000) in Western Blot

Tue Jul 28 00:00:00 UTC 2015

IL-21-mediated non-canonical pathway for IL-1β production in conventional dendritic cells.

Nature communications

Wan CK,Li P,Spolski R,Oh J,Andraski AB,Du N,Yu ZX,Dillon CP,Green DR,Leonard WJ

13-7000 was used in ChIP-seq to study IL-1beta production in conventional dendritic cells by IL-21-mediated non-canonical pathway

Thu Aug 13 00:00:00 UTC 2015

IL-21-mediated non-canonical pathway for IL-1β production in conventional dendritic cells.

Nature communications

Wan CK,Li P,Spolski R,Oh J,Andraski AB,Du N,Yu ZX,Dillon CP,Green DR,Leonard WJ

13-7000 was used in ChIP-seq to study IL-1beta production in conventional dendritic cells by IL-21-mediated non-canonical pathway

Thu Aug 13 00:00:00 UTC 2015

Dysregulated STAT1-SOCS1 control of JAK2 promotes mammary luminal progenitor cell survival and drives ERα(+) tumorigenesis.

Cell death and differentiation

Chan SR,Rickert CG,Vermi W,Sheehan KC,Arthur C,Allen JA,White JM,Archambault J,Lonardi S,McDevitt TM,Bhattacharya D,Lorenzi MV,Allred DC,Schreiber RD

13-7000 was used in EMSA to investigate the role of STAT1 in mammary luminal progenitor cell survival and tumorigenesis

Sat Feb 01 00:00:00 UTC 2014

Dysregulated STAT1-SOCS1 control of JAK2 promotes mammary luminal progenitor cell survival and drives ERα(+) tumorigenesis.

Cell death and differentiation

Chan SR,Rickert CG,Vermi W,Sheehan KC,Arthur C,Allen JA,White JM,Archambault J,Lonardi S,McDevitt TM,Bhattacharya D,Lorenzi MV,Allred DC,Schreiber RD

13-7000 was used in EMSA to investigate the role of STAT1 in mammary luminal progenitor cell survival and tumorigenesis

Sat Feb 01 00:00:00 UTC 2014

The cytokines IL-21 and GM-CSF have opposing regulatory roles in the apoptosis of conventional dendritic cells.

Immunity

Wan CK,Oh J,Li P,West EE,Wong EA,Andraski AB,Spolski R,Yu ZX,He J,Kelsall BL,Leonard WJ

Published figure using STAT3 monoclonal antibody (Product # 13-7000) in ChIP assay

Thu Mar 21 00:00:00 UTC 2013

BLIMP-1 and STAT3 counterregulate microRNA-21 during plasma cell differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Barnes NA,Stephenson S,Cocco M,Tooze RM,Doody GM

13-7000 was used in western blot to investigate microRNA expression during plasma cell differentiation.

Sun Jul 01 00:00:00 UTC 2012

Clinical and molecular studies of the effect of imatinib on advanced aggressive fibromatosis (desmoid tumor).

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

Heinrich MC,McArthur GA,Demetri GD,Joensuu H,Bono P,Herrmann R,Hirte H,Cresta S,Koslin DB,Corless CL,Dirnhofer S,van Oosterom AT,Nikolova Z,Dimitrijevic S,Fletcher JA

13-7000 was used in western blot to evaluate the clinical efficacy of imatinib in patients with advanced aggressive fibromatosis and investigate the mechanism

Wed Mar 01 00:00:00 UTC 2006

Src family kinases are important negative regulators of G-CSF-dependent granulopoiesis.

Blood

Mermel CH,McLemore ML,Liu F,Pereira S,Woloszynek J,Lowell CA,Link DC

13-7000 was used in EMSA to test if Src family kinases regulate granulocyte colony-stimulating factor receptor signaling

Sun Oct 15 00:00:00 UTC 2006

Ablation of oncogenic ALK is a viable therapeutic approach for anaplastic large-cell lymphomas.

Blood

Piva R,Chiarle R,Manazza AD,Taulli R,Simmons W,Ambrogio C,D'Escamard V,Pellegrino E,Ponzetto C,Palestro G,Inghirami G

13-7000 was used in western blot to examine the consequences of constitutive expression of anaplastic lymphoma kinase

Sun Jan 15 00:00:00 UTC 2006

HIV regulation of the IL-7R: a viral mechanism for enhancing HIV-1 replication in human macrophages in vitro.

Journal of leukocyte biology

Zhang M,Drenkow J,Lankford CS,Frucht DM,Rabin RL,Gingeras TR,Venkateshan C,Schwartzkopff F,Clouse KA,Dayton AI

Published figure using STAT3 monoclonal antibody (Product # 13-7000) in Western Blot

Thu Jun 01 00:00:00 UTC 2006

Malignant transformation but not normal cell growth depends on signal transducer and activator of transcription 3.

Cancer research

Schlessinger K,Levy DE

13-7000 was used in western blot to analyze the dependence on signal transducer and activator of transcription 3 of malignant transformation but not normal cell growth

Fri Jul 01 00:00:00 UTC 2005

PI3K-AKT pathway negatively controls EGFR-dependent DNA-binding activity of Stat3 in glioblastoma multiforme cells.

Oncogene

Ghosh MK,Sharma P,Harbor PC,Rahaman SO,Haque SJ

13-7000 was used in ChIP assay and western blot to elucidate how Stat3 and AKT provide survival signals for glioblastoma multiforme cells

Thu Nov 10 00:00:00 UTC 2005

Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.

Cancer research

Silver DL,Naora H,Liu J,Cheng W,Montell DJ

13-7000 was used in immunocytochemistry, immunohistochemistry - paraffin section, immunoprecipitation, and western blot to study the localization in focal adhesions and function in ovarian cancer cell motility of activated signal transducer and activator

Sat May 15 00:00:00 UTC 2004

Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs).

Oncogene

Duensing A,Medeiros F,McConarty B,Joseph NE,Panigrahy D,Singer S,Fletcher CD,Demetri GD,Fletcher JA

13-7000 was used in western blot to identify pathways that are constitutively activated in a KIT-dependent manner in gastrointestinal stromal tumors

Thu May 13 00:00:00 UTC 2004

Constitutive activation of Jak/STAT proteins in Epstein-Barr virus-infected B-cell lines from patients with posttransplant lymphoproliferative disorder.

Transplantation

Nepomuceno RR,Snow AL,Robert Beatty P,Krams SM,Martinez OM

13-7000 was used in immunoprecipitation to investigate signaling pathways in posttransplant lymphoproliferative disease.

Thu Aug 15 00:00:00 UTC 2002

Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as an hepatocyte-stimulating factor.

Proceedings of the National Academy of Sciences of the United States of America

Dumoutier L,Van Roost E,Colau D,Renauld JC

13-7000 was used in gel shift to clone and study human IL-TIF.

Tue Aug 29 00:00:00 UTC 2000

Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9.

Journal of immunology (Baltimore, Md. : 1950)

Dumoutier L,Louahed J,Renauld JC

13-7000 was used in gel shift to identify genes induced in T cells by IL-9 treatment.

Tue Feb 15 00:00:00 UTC 2000

Essential role of STAT3 for embryonic stem cell pluripotency.

Proceedings of the National Academy of Sciences of the United States of America

Raz R,Lee CK,Cannizzaro LA,d'Eustachio P,Levy DE

13-7000 was used in EMSA, immunoprecipitation, and western blot to examine the effect of STAT3 modulation using embryonic stem cells.

Tue Mar 16 00:00:00 UTC 1999

Essential role of STAT3 for embryonic stem cell pluripotency.

Proceedings of the National Academy of Sciences of the United States of America

Raz R,Lee CK,Cannizzaro LA,d'Eustachio P,Levy DE

13-7000 was used in EMSA, immunoprecipitation, and western blot to examine the effect of STAT3 modulation using embryonic stem cells.

Tue Mar 16 00:00:00 UTC 1999

ErbB-2 kinase is required for constitutive stat 3 activation in malignant human lung epithelial cells.

International journal of cancer

Fernandes A,Hamburger AW,Gerwin BI

13-7000 was used in western blot to elucidate ErbB-2/Her2/Neu signaling in the development of non-small-cell lung cancer.

Fri Nov 12 00:00:00 UTC 1999

Distinct roles for STAT1, STAT3, and STAT5 in differentiation gene induction and apoptosis inhibition by interleukin-9.

The Journal of biological chemistry

Demoulin JB,Van Roost E,Stevens M,Groner B,Renauld JC

13-7000 was used in gel shift to study IL-9 activation of STAT1, 3, and 5.

Fri Sep 03 00:00:00 UTC 1999

Distinct roles for STAT1, STAT3, and STAT5 in differentiation gene induction and apoptosis inhibition by interleukin-9.

The Journal of biological chemistry

Demoulin JB,Van Roost E,Stevens M,Groner B,Renauld JC

13-7000 was used in gel shift to study IL-9 activation of STAT1, 3, and 5.

Fri Sep 03 00:00:00 UTC 1999

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STAT3 Monoclonal Antibody (ST3-5G7)

STAT3 Antibody (13-7000) in ICC/IF

Product Details

13-7000

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

Gel Shift (GS)

Miscellaneous PubMed (MISC)

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Target Information

Performance Guarantee

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