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Progen
Vimentin Monoclonal Antibody (VIM 3B4)
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Product Details
65013PROGEN
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Amphibian,
Bovine,
Dog,
Chicken,
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
VIM 3B4
Immunogen
Vimentin (Purified From Bovine Lens)
Conjugate
Form
Liquid
Concentration
0.5 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reactivity: MW 57,000 intermediate filament protein (vimentin) of mesenchymal cells. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. Reactivity on cultured cell lines: fibroblasts (SV-80). This antibody was used in a Indirect ELISA.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis luminal protein 113; FLJ36605; RP11-124N14.1; VIME; Vimentin
Gene Aliases: BOS_12844; CTRCT30; HEL113; VIM
UniProt ID: (Bovine) P48616, (Human) P08670, (Dog) A0A8C0N8E3
Entrez Gene ID: (Bovine) 280955, (Chicken) 420519, (Human) 7431, (Dog) 477991
in utero embryonic development
biological_process
negative regulation of neuron projection development
astrocyte development
intermediate filament organization
Bergmann glial cell differentiation
lens fiber cell development
movement of cell or subcellular component
positive regulation of gene expression
viral process
muscle filament sliding
SMAD protein signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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