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11-Ketotestosterone Competitive ELISA Kit
Product Specifications
Analytical sensitivity
1.85 pg/mL
Assay range
8.2-2,000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Urine
50 µL
Fecal Extract
50 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<8%
Intraassay CV
<6%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
11-Ketotestosterone
Protein family
Chromone
Species (tested)
Chemical
Assay kit format
Competitive ELISA
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The multispecies 11-Ketotestosterone ELISA quantitates 11-Ketotestosterone in extracted serum, plasma, and dried fecal samples, or urine.
Principle of the method:
The 11-Ketotestosterone Competitive ELISA research-use-only kit is designed to quantitatively measure 11-Ketotestosterone independent of species. A 11-Ketotestosterone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with goat anti-rabbit IgG antibodies. 11-Ketotestosterone polyclonal rabbit antibodies and 11-Ketotestosterone-peroxidase conjugate is added to the wells. The 11-Ketotestosterone conjugate and any 11-Ketotestosterone in the sample will compete to bind to the 11-Ketotestosterone polyclonal rabbit antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound 11-Ketotestosteron-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of 11-Ketotestosterone present in the sample.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Androgenic hormones, such as testosterone, affect many male organisms’ growth, size, and reproduction. In teleost fish, along with testosterone, 11-ketotestosterone plays a significant role by inducing reproductive characteristics in both males and females. In particular male fish, 11-ketotestosterone levels increase during spermatogenesis in the spawning season, while in some female fish, 11-ketotestosterone increases before yolk deposition to regulate ovarian development.
The presence and involvement of 11-ketotestosterone in other species, such as humans, have only recently been established. In contrast to fish, primate serum 11-ketotestosterone concentrations were not significantly different in males and females, despite males having significantly higher circulating testosterone. This evidence suggests that 11-ketotestosterone production in these species may not be testis-dependent and primarily originates from adrenal-derived 11-oxyandrogen precursors. Recent studies have discovered more 11-ketotestosterone than its precursors, androstenedione, and testosterone, in prepubertal children and postmenopausal women compared to men. This discovery highlights the potential use of 11-ketotestosterone as a clinical biomarker to screen adrenal androgen excess in disease conditions such as polycystic ovary syndrome (PCOS) and hirsutism in women.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : 17-Hydroxyandrost-4-ene-3, 11-dione, 11-Ketotestosterone, 11 Oxotestosterone, 11-KT, 11KT
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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