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Invitrogen
Arg8-Vasopressin Competitive ELISA Kit
Product Specifications
Analytical sensitivity
0.88 pg/mL
Assay range
1.6-1,000 pg/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Tissue Homogenate
100 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<15%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
AVP
Protein family
Chromone
Assay kit format
Competitive ELISA
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The multispecies Arg8-Vasopressin (AVP) Competivite ELISA with quantitates Arg8-Vasopressin in serum, plasma, and tissue culture media.
Principle of the method:
The Arg8-Vasopressin (AVP) Competitive ELISA research-use-only kit is designed to quantitatively measure AVP independent of species. An AVP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with goat anti-rabbit IgG antibodies. Rabbit polyclonal antibodies and AVP-peroxidase conjugate is added to the wells. The AVP conjugate and any AVP in the sample will compete to bind to the rabbit polyclonal AVP antibodies. After 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of AVP present in the sample.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
This gene encodes a precursor protein consisting of arginine vasopressin and two associated proteins, neurophysin 2 and a glycopeptide, copeptin. Arginine vasopressin is a posterior pituitary hormone which is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : Vasopressin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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