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Product Specifications
Analytical sensitivity
0.25 ng/mL
Assay range
0.741-180 ng/mL
Sample type/volume
Plasma
50 µL
Supernatant
50 µL
Urine
12.5 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
7.6%
Intraassay CV
6.7%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
ANP
Species (tested)
Chemical
Assay kit format
Competitive ELISA
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Atrial Natriuretic Peptide (ANP) Competitive ELISA quantitates ANP in human plasma, urine or cell culture medium.
Principle of the method
The Atrial Natriuretic Peptide (ANP) Competitive ELISA research-use-only kit is designed to quantitatively measure ANP present in plasma, urine, and tissue culture media samples. This kit is not recommended for serum or plasma samples without extraction. An ANP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An ANP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to ANP to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound ANP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases : ANP, Pronatriodilatin, urodilatin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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