Search Thermo Fisher Scientific
Product Specifications
Analytical sensitivity
<1 pg/mL
Assay range
1.56-100 pg/mL
Sample type/volume
Cerebrospinal Fluid
25 µL
Supernatant
25 µL
Tissue Homogenate
25 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Interassay CV
7.5%
Intraassay CV
8.6%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Amyloid beta 42
Species (tested)
Human
Assay kit format
High Sensitivity Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Amyloid Beta 42 Ultrasensitive (Hu Abeta 42 US) ELISA quantitates Hu Abeta 42 at ultra-low levels in humantissue culture medium, tissue homogenates and cerebrospinal fluid. The assay will exclusively recognize both natural and recombinant Hu Abeta 42 US.
Principle of the method
The Human Abeta 42 US solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Beta amyloid is a 40-43 amino acid peptide cleaved from amyloid precursor protein by a protease, gamma-secretase. Beta Amyloid (1-40) (Amyloid beta 40 or Abeta 40) together with beta Amyloid (1-42) (Amyloid beta 42 or Abeta 42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the Amyloid beta 40 and Amyloid beta 42 alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : A4, AAA, ABETA, ABPP, AD1, APP, APPI, CTFgamma, CVAP, PN-II, PN2
Gene ID : (Human) 351
Gene symbol : APP
Protein Aliases : AB42, Aβ 1 42, B APP42, BAPP42, BetaAPP42, β APP42, βAPP42, A β 1 42, Amyloid, Amyloid b, Amyloid beta, Amyloid beta 1 42, Amyloid β, A beta 1 42, ABPP, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, amyloid beta (A4) precursor protein, Amyloid precursor protein, Amyloid-beta (A4) precursor protein, Amyloid-beta A4 protein, Amyloid-beta precursor protein, APP, APPI, beta-amyloid peptide, beta-amyloid peptide(1-40), beta-amyloid peptide(1-42), beta-amyloid precursor protein, Cerebral vascular amyloid peptide, CVAP, peptidase nexin-II, PN-II, PreA4, Protease nexin-II, testicular tissue protein Li 2
UniProt ID (Human) P05067
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support