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Invitrogen
Human BCAM ELISA Kit
Product Specifications
Analytical sensitivity
40 pg/mL
Assay range
40.96-10,000 pg/mL
Sample type/volume
Plasma
33 µL
Serum
33 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
BCAM
Protein family
Cell Structure/Organelle Proteins
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Basal Cell Adhesion Molecule (Hu BCAM) ELISA quantitates Hu BCAM in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu BCAM.
Principle of the method
The Human BCAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Lutheran blood group glycoprotein is a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five, N-terminus, extracellular immunoglobulin domains, a single transmembrane domain, and a short, C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AU, BCAM, CD239, LU, MSK19
Gene ID : (Human) 4059
Gene symbol : BCAM
Protein Aliases : antigen identified by monoclonal antibody F8, Auberger B antigen, B-CAM cell surface glycoprotein, B-cell adhesion molecule, Basal cell adhesion molecule, basal cell adhesion molecule (Lu and Au blood groups), basal cell adhesion molecule Lutheran blood group, CD239, F8/G253 antigen, glycoprotein 95kDa, Lutheran antigen, Lutheran blood group, Lutheran blood group glycoprotein, Lutheran blood group variant LUGA
UniProt ID (Human) P50895
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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