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Invitrogen
Human BMP-5 ELISA Kit
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Product Specifications
Analytical sensitivity
250 pg/mL
Assay range
0.246-60 ng/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
BMP5
Protein family
Growth Factors & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Bone Morphogenic Protein 5 (Hu BMP-5) ELISA quantitates Hu BMP-5 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu BMP-5.
Principle of the method
The Human BMP-5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
RB3 (Stathmin-4), encoded by STMN4 gene, is a neuron-specific homologue of the SCG10/stathmin family proteins, possessing a unique N-terminal membrane-associated domain and the stathmin-like domain at the C terminus, which promotes microtubule (MT) catastrophe and/or tubulin sequestering. Stathmin is a ubiquitous cytosolic phosphoprotein, preferentially expressed in the nervous system, and described as a relay integrating diverse intracellular signaling pathways. RB3 is consistently up-regulated in differentiated neuroblastoma cells, and stable expression of transfected RB3 could induce differentiation, suggesting a role of RB3 during neuroblastoma differentiation. Moreover, increased RB3 is a unique response protein in the stathmin-related proteins following optical nerve axotomy and RB3 may be a critical target for neurodegenerative diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : BMP5
Gene ID : (Human) 653
Gene symbol : BMP5
Protein Aliases : BMP-5, BMP 5, MGC34244, BMP, Bone morphogenetic protein, bone morphogenenic protein-5, Bone morphogenetic protein 5
UniProt ID (Human) P22003
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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