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Product Specifications
Analytical sensitivity
0.15 U/mL
Assay range
1-20 U/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
4.2%
Intraassay CV
3.2%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Incubation Buffer
HRP conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Standard
Standard Dilution Buffer
Incubation Buffer
HRP conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
CD23
Protein subtype
Lectins & Leptins
Protein family
CD Proteins
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human CD23 ELISA quantitates Hu CD23 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD23.
Principle of the method
The Human CD23 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : BLAST-2, CD23, CD23A, CLEC4J, FCE2, FCER2, IGEBF
Gene ID : (Human) 2208
Gene symbol : FCER2
Protein Aliases : BLAST-2, C-type lectin domain family 4 member J, C-type lectin domain family 4, member J, CD23, CD23 antigen, Fc epsilon receptor II, Fc fragment of IgE, low affinity II, receptor for (CD23), Fc-epsilon-RII, FceRII, Immunoglobulin E-binding factor, immunoglobulin epsilon-chain, Low affinity immunoglobulin epsilon Fc receptor, Lymphocyte IgE receptor
UniProt ID (Human) P06734
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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