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Invitrogen
Human CTX-II ELISA Kit
Product Specifications
Analytical sensitivity
0.1 ng/mL
Assay range
0.16-10 ng/mL
Sample type/volume
Serum
100 µL
Plasma
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
CTX-II
Protein family
Fibrillar collagen
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Cross Linked C-telopeptide of Type II Collagen (CTXII) ELISA quantitates CTXII in serum, plasma, and other biological fluids
The Human Cross Linked C-telopeptide of Type II Collagen (CTXII) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
CTX-II is a biomarker for type II collagen degradation, which is mainly found in cartilage. It is a small peptide fragment that is released into the synovial fluid and blood when there is cartilage degradation. CTX-II has been extensively studied as a diagnostic and prognostic marker for osteoarthritis, and it has also been shown to be a useful tool for monitoring the efficacy of treatments targeting cartilage degradation. The structure of CTX-II is a small peptide consisting of 17 amino acids. It is derived from the C-terminal telopeptide of type II collagen. CTX-II functions as a biomarker for cartilage degradation and can be used to monitor the progression of osteoarthritis and the effectiveness of treatments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : C-terminal cross-linked telopeptides of type II collagen, cartilage degradation marker, CTXII
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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