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Human Complement C4 ELISA Kit
Product Specifications
Analytical sensitivity
1.88 ng/mL
Assay range
3.13-200 ng/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Complement C4
Protein family
Complement Component
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Complement Component 4 (C4) ELISA quantitates C4 in serum, plasma, and other biological fluids
The Human Complement Component 4 (C4) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : C4, C4A, C4A2, C4A3, C4A4, C4A6, C4AD, C4B, C4B1, C4B12, C4B2, C4B3, C4B5, C4B_2, C4BD, C4F, C4S, CH, CO4, CPAMD2, CPAMD3, RG
Gene ID : (Human) 720, (Human) 721, (Human) 100293534
Gene symbol : C4A, C4B, C4B_2
Protein Aliases : acidic C4, Acidic complement C4, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3, C4A anaphylatoxin, Chido form of C4, Complement C4-A, Complement C4-B, complement C4B1a, DADB-112B14.11, DAMC-258G8.6, FLJ60561, MGC164979, c4 complement, MHC class III region complement, Rodgers form of C4
UniProt ID (Human) P0C0L4, (Human) P0C0L4, (Human) P0C0L5
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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