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Invitrogen
Human Complement Factor B ELISA Kit
Product Specifications
Analytical sensitivity
0.19 ng/mL
Assay range
0.31-20 ng/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Complement Factor B
Protein family
Complement Component
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Complement Factor B (CFB) ELISA quantitates Complement Factor B in serum, plasma, and other biological fluids
The Human Complement Factor B (CFB) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Factor B is a component of the alternative complement pathway. The protein is a single chain polypeptide with a molecular mass of 93 kDa. Factor B is cleaved by factor D into the Ba component (30 kDa) and the Bb (63 kDa). Bb together with C3b forms the alternative pathway C3 convertase C3bBb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AHUS4, ARMD14, BF, BFD, CFAB, CFB, CFBD, FB, FBI12, GBG, H2-Bf, PBF2
Gene ID : (Human) 629
Gene symbol : CFB
Protein Aliases : B-factor, properdin, C3 proaccelerator, C3 proactivator, C3/C5 convertase, Complement factor B, DADB-122G4.5, GBG, Glycine-rich beta glycoprotein, glycine-rich beta-glycoprotein, PBF2, Properdin factor B
UniProt ID (Human) P00751
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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