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Human Copeptin ELISA Kit
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Product Specifications
Analytical sensitivity
18.75 pg/mL
Assay range
31.25-2,000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Copeptin
Protein family
Vasopressin/oxytocin
Species (tested)
Human
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Copeptin (CPP) ELISA quantitates CPP in serum, plasma and other biological fluids
Principle of the method:
The Competitive Copeptin (CPP) ELISA research-use-only kit is designed to quantitatively measure CPP in Humans. A CPP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human CPP is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human CPP in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Copeptin is a member of vasopressin/oxytocin family. It is a 39-amino acid peptide produced from cleavage of preprovasopressin during precursor processing, and is released along with the hormone arginine vasopressin. Copeptin acts to ensure the proper structural formation of arginine vasopressin during precursor processing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : ADH, ARVP, AVP, AVP-NPII, AVRP, VP
Gene ID : (Human) 551
Gene symbol : AVP
Protein Aliases : antidiuretic hormone, AVP-NPII, copeptin, neurohypophyseal, prepro-arginine-vasopressin-neurophysin II, prepro-AVP-NP II, Vasopressin-neurophysin 2-copeptin, vasopressin-neurophysin II-copeptin
UniProt ID (Human) P01185
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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