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Human Cytochrome C Matched Antibody Pair
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Product Specifications
Analytical sensitivity
0.08 ng/mL
Assay range
0.08-5 ng/mL
Sample type/volume
Cell Lysate
2 µL
Plasma
10 µL
Serum
50 µL
Hands-on time
3 hr 10 min
Time-to-result
24 hr
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
10 x 96 Tests
Contents
Capture (coating) Antibody
Detection Antibody
Standard
SAV-HRP
Assay Buffer
Lysis Buffer
Detection Antibody
Standard
SAV-HRP
Assay Buffer
Lysis Buffer
Shipping conditions
Wet ice
Storage
-20°C
Protein name
Cytochrome C
Species (tested)
Human
Assay kit format
Matched Antibody Pair
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Cytochrome C Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, assay buffer, lysis buffer and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human Cytochrome C. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CYC, CYCS, HCS, THC4
Gene ID : (Human) 54205
Gene symbol : CYCS
Protein Aliases : CRF, hc1, Cytochrome c
UniProt ID (Human) P99999
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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