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Product Specifications
Analytical sensitivity
10.6 pg/mL
Assay range
25-2000 pg/mL
Sample type/volume
Serum
25 µL
Plasma
25 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr
Homogenous (no wash)
No
Interassay CV
8.9%
Intraassay CV
11%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well strip-well plate
Standard
Controls
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Standard
Controls
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Estradiol
Protein family
Hormones & Receptors
Species (tested)
Human
Species (reported)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human Estradiol E2 ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA). This assay is designed to detect and quantify the level of 17-beta-estradiol (E2) in serum and plasma. The assay will recognize both natural and recombinant human estradiol E2.
Principle of the method
The Human Estradiol solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Estradiol (17 beta-estradiol) is a sex hormone. Estradiol is the predominant sex hormone present in females. It is also present in males, being produced as an active metabolic product of testosterone. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including the bones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : 17-Beta-Estradiol, 17-beta-HSD
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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