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          • ELISA Kits ›
          • IL-12 p40 ELISA Kits

          Invitrogen

          Human IL-12 p40 Matched Antibody Pair

          3 References
          View all (6) IL-12 p40 ELISA Kits

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          Technical guide & protocol Tech Support
          Technical guide & protocol Tech Support
          Technical guide & protocol Tech Support
          Human IL-12 p40 Matched Antibody Pair
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          Human IL-12 p40 Matched Antibody Pair
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          Human IL-12 p40 Matched Antibody Pair

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          Human IL-12 p40 Matched Antibody Pair
          Human IL-12 p40 Matched Antibody Pair (CHC1563)
          videoThumbnail
          ►

          Product Specifications

          Analytical sensitivity

          <31.2 pg/mL

          Assay range

          31.2-2,000 pg/mL

          Sample type/volume

          Plasma
          100 µL
          Serum
          100 µL
          Supernatant
          100 µL

          Hands-on time

          1 hr 30 min

          Time-to-result

          24 hr

          Homogenous (no wash)

          No

          Instrument

          Absorbance Microplate Reader

          Product size

          10 x 96 Tests

          Contents

          Capture (coating) Antibody
          Detection Antibody
          Standard
          Streptavidin-HRP

          Shipping conditions

          Wet ice

          Storage

          2-8°C

          Protein name

          IL-12 p40

          Species (tested)

          Human

          Assay kit format

          Matched Antibody Pair

          Detector antibody conjugate

          Biotin

          Label or dye

          HRP

          About This Kit

          The Human Interleukin 12 p40 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-12 p40. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.

          Principle of the method

          ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

          Target information

          IL12B (IL-12 p40) encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kDa cytokine receptor like subunit encoded by this gene, and a 35 kDa subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Gene aliases : CLMF, CLMF2, IL-12B, IL12B, IMD28, IMD29, NKSF, NKSF2

          Gene ID : (Human) 3593

          Gene symbol : IL12B

          Protein Aliases : CLMF p35, IL-12 subunit p35, IL12A, IL12 p40, ILN, Interleukin, Interleukin 12, Interleukin12, il 12, IL-12A, NKSF1, RP23-388G23.1, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, IL12, subunit p40, interleukin 12, p40, interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), interleukin-12 beta chain, Interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD subunit, NK cell stimulatory factor chain 2, NKSF2

          UniProt ID (Human) P29460

          Recommended Products

          IL-6 Human Matched Antibody Pair

          Because you are interested in IL-6 Human ELISA Kit.

          SIZE

          10 x 96 tests

          PRICE

          USD 400.00

          Cat # CHC1263

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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