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Human Luteinizing Hormone ELISA Kit
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Product Specifications
Analytical sensitivity
140 pg/mL
Assay range
156.3-10,000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Luteinizing Hormone
Protein family
Other Proteins
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Luteinizing Hormone (Hu LH) ELISA quantitates Hu LH in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu LH.
Principle of the method
The Human LH solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
LH is a member of the glycoprotein hormone family, Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : CG-ALPHA, CGA, CGB4, FSHA, GPHa, GPHA1, HCG, HH23, LHA, LHB, LSH-B, LSH-beta, TSHA
Gene ID : (Human) 1081, (Human) 3972
Gene symbol : CGA, LHB
Protein Aliases : Anterior pituitary glycoprotein hormones common subunit alpha, CG-alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, chorionic gonadotropin, alpha polypeptide, Follicle-stimulating hormone alpha chain, follicle-stimulating hormone alpha subunit, Follitropin alpha chain, FSH-alpha, Glycoprotein hormones alpha chain, interstitial cell stimulating hormone, beta chain, LH-B, LSH-alpha, Luteinizing hormone alpha chain, luteinizing hormone beta subunit, Luteinizing hormone subunit beta, Lutropin alpha chain, Lutropin beta chain, LSH-B, LSH-beta, hLHB, follitropin, FSH-B, FSH-beta, Lutropin subunit beta, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, TSH-alpha
UniProt ID (Human) P01215, (Human) P01229
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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