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Invitrogen
Human MR-ProADM ELISA Kit
Product Specifications
Analytical sensitivity
9.38 pg/mL
Assay range
15.63-1,000 pg/mL
Sample type/volume
Tissue Homogenate
100 µL
Cell Lysate
100 µL
Serum
100 µL
Plasma
100 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
MR-proADM
Species (tested)
Human
Species (reported)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Mid regional pro adrenomedullin ELISA Kit (MR ProADM) ELISA quantitates MR ProADM in serum, plasma and other biological fluids.
Principle of the method
The Human Mid regional pro adrenomedullin (MR ProADM) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
MR-proADM, short for mid-regional proadrenomedullin, is a precursor protein that gives rise to the adrenomedullin (ADM) hormone. It is involved in various physiological processes, particularly in the regulation of cardiovascular function and inflammation. Functionally, MR-proADM is associated with vasodilation, blood pressure regulation, and immune response modulation. It acts as a biomarker for endothelial dysfunction and can be used to assess the severity of certain cardiovascular diseases. Elevated levels of MR-proADM have also been linked to systemic inflammation and sepsis. Structurally, MR-proADM is a protein that consists of a chain of amino acids. It undergoes post-translational processing to generate the active form of ADM, which is a shorter peptide consisting of 52 amino acids. Localization-wise, MR-proADM is primarily produced in various tissues, including the adrenal glands, lungs, and endothelial cells lining blood vessels. It is released into the bloodstream and can be measured as a biomarker to provide insight into cardiovascular health and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : ADM, AM, PAMP
Gene ID : (Human) 133
Gene symbol : ADM
Protein Aliases : Mid-regional proadrenomedullin, preproadrenomedullin, Pro-adrenomedullin, proadrenomedullin N-20 terminal peptide
UniProt ID (Human) P35318
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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