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Invitrogen
Human Nesfatin-1 ELISA Kit
Product Specifications
Analytical sensitivity
0.25 ng/mL
Assay range
0.25-60 ng/mL
Sample type/volume
Supernatant
50 µL
Serum
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
NUCB2
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human Nesfatin-1 quantitates human Nesfatin-1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Nesfatin-1.
Target information
NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : HEL-S-109, NEFA, NUCB2
Gene ID : (Human) 4925
Gene symbol : NUCB2
Protein Aliases : DNA-binding protein NEFA, Epididymis secretory protein Li 109, Gastric cancer antigen Zg4, nesfatin 1, nesfatin-1, Nucleobindin-2, Nucleobindin-2/Nesfatin-1, nucleobinding 2, prepronefastin, Prepronesfatin
UniProt ID (Human) P80303
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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