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Invitrogen
Human Nociceptin/Orphanin FQ ELISA Kit
Product Specifications
Analytical sensitivity
2.81 pg/mL
Assay range
4.69-300 pg/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Tissue Homogenate
100 µL
Cell Lysate
100 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Nociceptin/Orphanin FQ
Species (tested)
Human
Species (reported)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Orphanin FQ/Nociceptin (OFQ/N) ELISA quantitates OFQ/N in serum, plasma and other biological fluids.
Principle of the method
The Human Orphanin FQ/Nociceptin (OFQ/N) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Nociceptin/orphanin FQ is a neuropeptide that acts as a ligand for the nociceptin receptor. It modulates pain perception, stress responses, and various physiological processes. Structurally, it is a 17-amino acid peptide derived from the prepronociceptin protein with the sequence FGGFTGARKSARKLANQ. Nociceptin/orphanin FQ is widely distributed in the central nervous system and peripheral tissues, indicating its involvement in diverse functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : N/OFQ, OFQ/N
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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