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Product Specifications
Analytical sensitivity
10 pg/mL
Assay range
10.24-2,500 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
PDGF-AB
Protein family
Growth Factors & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Platelet Derived Growth Factor-AB (Hu PDGFAB) ELISA quantitates Hu PDGFAB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PDGFAB.
Principle of the method
The Human PDGFAB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-alpha, TGF-beta and EGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : c-sis, IBGC5, PDGF-2, PDGF-A, PDGF1, PDGF2, PDGFA, PDGFB, SIS, SSV
Gene ID : (Human) 5154, (Human) 5155
Gene symbol : PDGFA, PDGFB
Protein Aliases : becaplermin, H-PDGF-AB, PDGFAB, PDGF A-chain, PDGF subunit A, PDGF subunit B, PDGF, B chain, PDGF-1, PDGF-2, platelet-derived growth factor 2, Platelet-derived growth factor A chain, platelet-derived growth factor A-chain, platelet-derived growth factor alpha chain, Platelet-derived growth factor alpha polypeptide, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog), Platelet-derived growth factor subunit A, Platelet-derived growth factor subunit B, platelet-derived growth factor, beta polypeptide (oncogene SIS), Proto-oncogene c-Sis
UniProt ID (Human) P04085, (Human) P01127
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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