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Product Specifications
Analytical sensitivity
13.4 pg/mL
Assay range
39.1-2500 pg/mL
Sample type/volume
Saliva
10 µL
Serum
10 µL
Supernatant
100 µL
Urine
10 µL
Whole Blood
10 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 45 min
Homogenous (no wash)
No
Interassay CV
4%
Intraassay CV
10.7%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent
Detection Antibody
AP Conjugate
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent
Detection Antibody
AP Conjugate
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Dry ice
Storage
-20°C
Protein name
PTGES2
Protein family
Cytokines & Receptors
Species (tested)
Human
Assay kit format
Competitive ELISA
Detector antibody conjugate
AP
Label or dye
AP
About This Kit
The Human Prostaglandin E2 (Hu PGE2) ELISA quantitates Hu PGE2 in human serum, whole blood, saliva, urine or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PGE2.
Principle of the method
The Human PGE2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Prostoglandin E2 (PGE2) is a potent lipid mediator produced by metabolism of arachidonic acid via the cyclooxygenase (COX) pathway. Following cellular stimulation, arachidonic acid is hydrolyzed from phospholipids stores by one of a family of phospholipase A2 enzymes and converted to the unstable endoperoxide PGH2 by either COX 1 or COX 2. PGH2 is isomerized to PGE2 by one of 3 distinct enzymes microsomal PGES 1 (mPGES 1), mPGES 2, or a cytosolic enzyme, cPGES. PGE2 is active in a number of physiological systems and settings including inflammation, immune regulation, generation of fever, pain perception, protection of the gastric muscosa, fertility and parturition, as well as sodium and water retention. The effects of PGE2 are transduced by 4 subtypes of G protein coupled receptors designated EP1, EP2, EP3, EP4, with Kd values for PGE2 ranging from 1 10 nM. PGE2 is rapidly metabolized in vivo by the prostaglandin 15 dehydrogenase pathway (15 hydroxy PGDH) to the inactive metabolite 13,14 dihydro 15 keto PGE2. The half life of PGE2 in the circulatory system is ~30 seconds and normal plasma levels are 3 12 pg/mL
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : C9orf15, GBF-1, GBF1, mPGES-2, PGES2, PTGES2
Gene ID : (Human) 80142
Gene symbol : PTGES2
Protein Aliases : C20H32O5, PGE2, FLJ14038, MGC11289, RP11-395P17.2, gamma-interferon-activated transcriptional element-binding factor 1, GATE-binding factor 1, membrane-associated prostaglandin E synthase 2, Membrane-associated prostaglandin E synthase-2, Microsomal prostaglandin E synthase 2, microsomal prostaglandin E synthase-2, mPGE synthase-2, mPGES-2, Prostaglandin E synthase 2, Prostaglandin-H(2) E-isomerase
UniProt ID (Human) Q9H7Z7
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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