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Product Specifications
Assay range
31.3-4,000 pg/mL
Sample type/volume
Plasma
100 µL
Urine
100 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
11.7%
Intraassay CV
6%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Tris Buffer
Alkaline Phosphatase Tracer
Leukotriene B4 Antiserum
DEA Buffer
Wash Buffer
pNPP tablets
Adhesive Plate Covers
Standard
Tris Buffer
Alkaline Phosphatase Tracer
Leukotriene B4 Antiserum
DEA Buffer
Wash Buffer
pNPP tablets
Adhesive Plate Covers
Shipping conditions
Dry ice
Storage
Store kit at -20°C. Refer to product documentation for component specific storage temperature.
Protein name
PTGES2
Species (tested)
Human
Assay kit format
Competitive ELISA
Label or dye
AP
About This Kit
The Human Prostaglandin E2 ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (ELISA). This assay is designed to detect and quantify the level of human prostaglandin E2 in biological samples.
Principle of the method
This assay is based on the competition between Prostaglandin E 2 (PGE2) and a PGE2 alkaline phosphatase tracer for a limited amount of PGE2 specific monoclonal antibody. Because of the competition between PGE2 in the sample and PGE2 tracer for the PGE2 antibody, the signal obtained with the assay will be inversely proportional to the amount of PGE 2 in each sample.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Prostoglandin E2 (PGE2) is a potent lipid mediator produced by metabolism of arachidonic acid via the cyclooxygenase (COX) pathway. Following cellular stimulation, arachidonic acid is hydrolyzed from phospholipids stores by one of a family of phospholipase A2 enzymes and converted to the unstable endoperoxide PGH2 by either COX 1 or COX 2. PGH2 is isomerized to PGE2 by one of 3 distinct enzymes microsomal PGES 1 (mPGES 1), mPGES 2, or a cytosolic enzyme, cPGES. PGE2 is active in a number of physiological systems and settings including inflammation, immune regulation, generation of fever, pain perception, protection of the gastric muscosa, fertility and parturition, as well as sodium and water retention. The effects of PGE2 are transduced by 4 subtypes of G protein coupled receptors designated EP1, EP2, EP3, EP4, with Kd values for PGE2 ranging from 1 10 nM. PGE2 is rapidly metabolized in vivo by the prostaglandin 15 dehydrogenase pathway (15 hydroxy PGDH) to the inactive metabolite 13,14 dihydro 15 keto PGE2. The half life of PGE2 in the circulatory system is ~30 seconds and normal plasma levels are 3 12 pg/mL
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : C9orf15, GBF-1, GBF1, mPGES-2, PGES2, PTGES2
Gene ID : (Human) 80142
Gene symbol : PTGES2
Protein Aliases : C20H32O5, PGE2, FLJ14038, MGC11289, RP11-395P17.2, gamma-interferon-activated transcriptional element-binding factor 1, GATE-binding factor 1, membrane-associated prostaglandin E synthase 2, Membrane-associated prostaglandin E synthase-2, Microsomal prostaglandin E synthase 2, microsomal prostaglandin E synthase-2, mPGE synthase-2, mPGES-2, Prostaglandin E synthase 2, Prostaglandin-H(2) E-isomerase
UniProt ID (Human) Q9H7Z7
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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