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Human RBP4 ELISA Kit
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Product Specifications
Analytical sensitivity
0.034 ng/mL
Assay range
0.063-4 ng/mL
Sample type/volume
Cell Lysate
10 µL
Plasma
10 µL
Serum
10 µL
Supernatant
10 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
8.1%
Intraassay CV
5%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
RBP4
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Retinol-binding protein 4 (HuRBP-4) ELISA quantitates Hu RBP-4 in human serum, plasma, cell lysates, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu RBP-4.
Principle of the method
The Human RBP-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
This protein belongs to the lipocalin family and is the specific carrier for retinol in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein post translationally and results in defective delivery and supply to the epidermal cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : MCOPCB10, PRO2222, RBP4, RDCCAS
Gene ID : (Human) 5950
Gene symbol : RBP4
Protein Aliases : Plasma retinol-binding protein, PRBP, RBP, retinol, retinol binding, Plasma retinol binding protein 4 (PRBP), Retinol binding protein 4 interstitial, retinol binding protein 4, plasma, Retinol-binding protein 4, retinol-binding protein 4, interstitial
UniProt ID (Human) P02753
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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