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Product Specifications
Analytical sensitivity
0.1 ng/mL
Assay range
2-142 ng/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 15 min
Homogenous (no wash)
No
Interassay CV
9.2%
Intraassay CV
2.9%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Incubation Buffer
HRP conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Standard
Standard Dilution Buffer
Incubation Buffer
HRP conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
TNFR2
Target sub-specificity
Soluble
Protein family
Cytokines & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Tumor Necrosis Factor Receptor II (Hu TNFRII) ELISA quantitates Hu TNFR1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNFRII.
Principle of the method
The Human TNFRII solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : CD120b, p75, p75TNFR, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, TNFRSF1B
Gene ID : (Human) 7133
Gene symbol : TNFRSF1B
Protein Aliases : CD120b, p75, p75 TNF receptor, p80 TNF-alpha receptor, sCD120b, soluble CD120b, soluble TNFR2, sTNF R2, sTNFR 2, sTNFR2, TNFR, TNFR II, Tumor necrosis factor receptor, soluble TNFR1B variant 1, TNF-R2, TNF-RII, tumor necrosis factor beta receptor, tumor necrosis factor binding protein 2, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, tumor necrosis factor receptor superfamily, member 1B, Tumor necrosis factor receptor type II
UniProt ID (Human) P20333
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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