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Invitrogen
Human proGRP ELISA Kit
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Product Specifications
Analytical sensitivity
9.38 pg/mL
Assay range
15.63-1,000 pg/mL
Sample type/volume
Cell Lysate
100 µL
Tissue Homogenate
100 µL
Plasma
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
proGRP
Species (tested)
Human
Species (reported)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Pro Gastrin Releasing Peptide (ProGRP) ELISA quantitates ProGRP in serum, plasma and other biological fluids.
Principle of the method
The Human ProGRP solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
ProGRP, short for Pro-gastrin-releasing peptide, is a precursor protein that gives rise to the gastrin-releasing peptide (GRP) hormone. It plays a role in various physiological processes, particularly in the regulation of neuroendocrine functions. Functionally, proGRP is involved in the control of cell growth, neurotransmission, and the regulation of the release of gastrointestinal hormones. It acts as a neuropeptide and can influence the secretion of gastrin and other gastrointestinal peptides. Additionally, proGRP has been implicated in the development and progression of certain cancers, particularly small cell lung cancer. Structurally, proGRP is a protein that consists of a chain of amino acids. It undergoes post-translational processing to generate the active form of GRP, which is a shorter peptide consisting of 27 amino acids. Localization-wise, proGRP is primarily produced in the nervous system, particularly in neurons of the central and peripheral nervous systems. It is also found in certain endocrine cells of the gastrointestinal tract and in some neuroendocrine tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : BN, GRP, GRP-10, preproGRP, proGRP
Gene ID : (Human) 2922
Gene symbol : GRP
Protein Aliases : bombesin, Gastrin-releasing peptide, GRP, neuromedin C, pre-progastrin releasing peptide, prepro-GRP, pro-grp, testicular tissue protein Li 103
UniProt ID (Human) P07492
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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