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Invitrogen
Mouse BSSP 4 (PRSS22) ELISA Kit
Product Specifications
Analytical sensitivity
60 pg/mL
Assay range
62.5-4,000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
BSSP4
Protein family
Proteases Peptidases & Inhibitors
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Mouse BSSP 4 (PRSS22) ELISA quantitates Ms BSSP 4 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms BSSP 4.
Principle of the method
The Mouse BSSP 4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
This gene encodes a member of the trypsin family of serine proteases. The enzyme is expressed in the airways in a developmentally regulated manner. The gene is part of a cluster of serine protease genes on chromosome 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : 4733401N09Rik, BSSP-4, Bssp4, Prss22, Prss26, SP001LA
Gene ID : (Mouse) 70835
Gene symbol : Prss22
Protein Aliases : Brain-specific serine protease 4, BSSP-4, Serine protease 22, Serine protease 26, Tryptase epsilon, BSP-2, MGC9599, protease, serine, 22
UniProt ID (Mouse) Q9ER10
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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