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Product Specifications
Analytical sensitivity
0.1 ng/mL
Assay range
0.1-24 ng/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Apolipoprotein J
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Mouse Clusterin quantitates mouse Clusterin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse Clusterin.
Target information
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AI893575, ApoJ, Cli, Clu, D14Ucla3, Msgp-2, Sgp-2, Sgp2, SP-40, Sugp-2
Gene ID : (Mouse) 12759
Gene symbol : Clu
Protein Aliases : Apo-J, Apolipoprotein J, Clusterin, Clustrin, complement lysis inhibitor, HGNC:2095, MGC24903, Clusterin alpha / beta chain, Complement associated protein SP40, GP80, Ku70-binding protein 1 (KUB1), Sulfated glycoprotein 2 (SGP2), Testosterone repressed prostate message 2 (TPRM2B), ApoJ β, ApoJa, ApoJb, apo j, Aging associated protein 4, Apo Jb, ApoJ a, ApoJ alpha, ApoJ b, ApoJ beta, ApoJ α, Apo J alpha, Apo J b, Apo J beta, Apo J α, Apo J β, Apo Ja, Apo J a, SGP-2, Sulfated glycoprotein 2, testosterone repressed prostate message, testosterone repressed prostate message-2
UniProt ID (Mouse) Q06890
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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